Activities per year
The mycotoxin lolitrem B is found in endophyte-infected perennial ryegrass (Lolium perenne) and is present in around 90% of ryegrass pastures in Australia and New Zealand. Ingestion causes a neurological syndrome in grazing livestock called Perennial Rye Grass Toxicosis (PRGT) (Cunningham, 1959; Combs et al., 2014) where clinical signs include hyperexcitability, muscle tremors, ataxia (“staggers”) and, in severe cases, clonic seizures and death (Combs et al., 2014). It remains unclear whether these neurological signs result only from the blockade of large conductance potassium channels (BK channels) by indole diterpenoid toxins, primarily lolitrem B (Munday-Finch SC., 1997; Imlach, 2011). Furthermore, the toxicity of biosynthetic pathway intermediates of lolitrem B is also largely undefined. To address these questions, lolitrem B and several intermediates were purified from infected perennial ryegrass. Using a time-series analysis, the effects on movement in dosed mice were quantified for lolitrem B and related compounds. Animals exposed to lolitrem B showed a significant increase in tremor and decreased ability to maintain stability during accelerated rotarod testing, while the other metabolites showed varied effects. Metabolic profiling of the mice tissues and serum were undertaken for animals exposed to lolitrem B at key time points. This study suggests the possible mechanism and quantity of residual lolitrem B in tissue samples. Characterization of clinical signs and metabolic changes associated with exposure to the family of indole diterpenoid toxins present in perennial ryegrass will provide useful information for generation of animal safe grass varieties for the dairy and meat industries.
|Number of pages||1|
|Publication status||Published - 2017|
|Event||Metabolomics 2017: 13th Annual Conference of the Metabolomics Society - Brisbane Convention and Exhibition Centre, Brisbane, Australia|
Duration: 25 Jun 2017 → 29 Jun 2017
|Period||25/06/17 → 29/06/17|
FingerprintDive into the research topics of 'Perennial ryegrass toxicosis: effects of the indole diterpenoid mycotoxins on movement and metabolism in a mouse model'. Together they form a unique fingerprint.
- 1 Internal HDR Supervision
Clinical and Pathological Characterisation of Perennial Ryegrass Toxicosis and Investigation of Bromide as a Therapeutic Agent.01 Feb 2010 → 31 Aug 2020
Activity: Supervision/Examination/Mentoring › Internal HDR Supervision