Peripheral biomarkers of oxidative stress in dogs with acute pancreatitis

Nicole V Tusa, Angel Abuelo, Nyssa A Levy, Jeffery C Gandy, Daniel K Langlois, Harry Cridge

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
48 Downloads (Pure)

Abstract

Background

Oxidative stress is considered a pathomechanism of acute pancreatitis (AP), but no studies have extensively characterized oxidant status in dogs with naturally-occurring AP.

Hypothesis or Objectives

Evaluate measures of oxidant status in dogs with AP and explore whether these measures correlate with AP severity.

Animals

Fifteen dogs with AP and 9 control dogs.

Methods

Prospective, controlled observational study. Plasma reactive metabolite (RM) concentrations, antioxidant potential (AOP), and urinary F2 isoprostane concentrations were measured in AP dogs and healthy controls. Severity of AP was assessed by length of hospitalization and 3 disease severity indices: canine acute pancreatitis severity (CAPS), modified canine activity index (M-CAI), and the acute patient physiologic and laboratory evaluation score (APPLEfull).

Results

Reactive metabolite (RM) concentrations (median, 65 relative fluorescent units [RFU]/μL; range, 20-331 RFU/μL) and RM:AOP (median, 7; range, 4-109) were higher in AP dogs than healthy controls (median RM, 25 RFU/μL; range, 16-41 RFU/μL; median RM:AOP, 4; range, 2-7; P < .001 for both comparisons). Reactive metabolite (rS = 0.603, P = .08) and RM:AOP (rS = 0.491, P = .06) were not correlated with the duration of hospitalization or disease severity indices evaluated. However, disease severity indices did not predict mortality in our study. Normalized urine 2,3-dinor-8-iso-prostaglandin F2α concentrations were correlated with C-reactive protein (CRP; rS = 0.491, P = .03), canine specific pancreatic lipase (Spec cPL; rS = 0.746, P = .002), and CAPS (rS = 0.603, P = .02).

Conclusions and Clinical Importance

Oxidant status is altered in dogs with naturally occurring AP, but the clinical relevance of this finding is unknown.

Original languageEnglish
Pages (from-to)1958-1965
Number of pages8
JournalJournal of Veterinary Internal Medicine
Volume36
Issue number6
Early online date10 Sept 2022
DOIs
Publication statusPublished - 01 Nov 2022

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