Peroxisome aggregation during cytokinesis in different angiosperm taxa

David Collings, John Harper

Research output: Contribution to journalArticle

3 Citations (Scopus)
10 Downloads (Pure)

Abstract

During cytokinesis in onion (Allium cepa L.) and leek (Allium porrum L.), all the peroxisomes present within the dividing cell aggregate in the phragmoplast adjacent to the developing cell plate. In order to understand the functional implications of this novel arrangement, especially in onion, which has hitherto been regarded as a model system in which to study plant cell division, we investigated how widespread the phenomenon was in selected monocots and dicots. During monocot cytokinesis, peroxisomes lacked aggregation in some taxa, notably grasses, and underwent partial aggregation in other taxa. However, complete aggregation of a cell's entire complement of peroxisomes was restricted to the genus Allium. Although peroxisomal aggregation has been suggested to function in the formation of the cell plate, the distribution of partial and complete aggregation in monocots did not match known differences in primary cell wall biochemistry. No aggregation was seen during cytokinesis in dicots. Through quantification of peroxisome distribution in cells whose actin microfilaments were disrupted with either latrunculin or cytochalasin, we demonstrated that peroxisomal aggregation is a microfilament-dependent process in Allium and the closely related plant Tristagma, which shows only partial aggregation. We speculate whether analysis of the peroxisomal proteome might reveal novel function(s) for aggregated peroxisomes during cytokinesis
Original languageEnglish
Pages (from-to)241-252
Number of pages12
JournalInternational Journal of Plant Sciences
Volume169
Issue number2
DOIs
Publication statusPublished - Feb 2008

Fingerprint

cytokinesis
peroxisomes
angiosperm
Angiospermae
Liliopsida
Allium
Magnoliopsida
microfilaments
onions
cytochalasins
cells
Allium porrum
leeks
Allium cepa
cell aggregates
proteome
biochemistry
cell division
complement
cell walls

Cite this

@article{277a71551b464b6a90545d6b654f2780,
title = "Peroxisome aggregation during cytokinesis in different angiosperm taxa",
abstract = "During cytokinesis in onion (Allium cepa L.) and leek (Allium porrum L.), all the peroxisomes present within the dividing cell aggregate in the phragmoplast adjacent to the developing cell plate. In order to understand the functional implications of this novel arrangement, especially in onion, which has hitherto been regarded as a model system in which to study plant cell division, we investigated how widespread the phenomenon was in selected monocots and dicots. During monocot cytokinesis, peroxisomes lacked aggregation in some taxa, notably grasses, and underwent partial aggregation in other taxa. However, complete aggregation of a cell's entire complement of peroxisomes was restricted to the genus Allium. Although peroxisomal aggregation has been suggested to function in the formation of the cell plate, the distribution of partial and complete aggregation in monocots did not match known differences in primary cell wall biochemistry. No aggregation was seen during cytokinesis in dicots. Through quantification of peroxisome distribution in cells whose actin microfilaments were disrupted with either latrunculin or cytochalasin, we demonstrated that peroxisomal aggregation is a microfilament-dependent process in Allium and the closely related plant Tristagma, which shows only partial aggregation. We speculate whether analysis of the peroxisomal proteome might reveal novel function(s) for aggregated peroxisomes during cytokinesis",
keywords = "Open access version available, Actin microfilaments, Allium, Cell division, Microtubules, Peroxisomes, Phragmoplast",
author = "David Collings and John Harper",
note = "Imported on 12 Apr 2017 - DigiTool details were: month (773h) = February 2008; Journal title (773t) = International Journal of Plant Sciences. ISSNs: 1058-5893;",
year = "2008",
month = "2",
doi = "10.1086/523965",
language = "English",
volume = "169",
pages = "241--252",
journal = "Botanical Gazette",
issn = "1058-5893",
publisher = "University of Chicago",
number = "2",

}

Peroxisome aggregation during cytokinesis in different angiosperm taxa. / Collings, David; Harper, John.

In: International Journal of Plant Sciences, Vol. 169, No. 2, 02.2008, p. 241-252.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Peroxisome aggregation during cytokinesis in different angiosperm taxa

AU - Collings, David

AU - Harper, John

N1 - Imported on 12 Apr 2017 - DigiTool details were: month (773h) = February 2008; Journal title (773t) = International Journal of Plant Sciences. ISSNs: 1058-5893;

PY - 2008/2

Y1 - 2008/2

N2 - During cytokinesis in onion (Allium cepa L.) and leek (Allium porrum L.), all the peroxisomes present within the dividing cell aggregate in the phragmoplast adjacent to the developing cell plate. In order to understand the functional implications of this novel arrangement, especially in onion, which has hitherto been regarded as a model system in which to study plant cell division, we investigated how widespread the phenomenon was in selected monocots and dicots. During monocot cytokinesis, peroxisomes lacked aggregation in some taxa, notably grasses, and underwent partial aggregation in other taxa. However, complete aggregation of a cell's entire complement of peroxisomes was restricted to the genus Allium. Although peroxisomal aggregation has been suggested to function in the formation of the cell plate, the distribution of partial and complete aggregation in monocots did not match known differences in primary cell wall biochemistry. No aggregation was seen during cytokinesis in dicots. Through quantification of peroxisome distribution in cells whose actin microfilaments were disrupted with either latrunculin or cytochalasin, we demonstrated that peroxisomal aggregation is a microfilament-dependent process in Allium and the closely related plant Tristagma, which shows only partial aggregation. We speculate whether analysis of the peroxisomal proteome might reveal novel function(s) for aggregated peroxisomes during cytokinesis

AB - During cytokinesis in onion (Allium cepa L.) and leek (Allium porrum L.), all the peroxisomes present within the dividing cell aggregate in the phragmoplast adjacent to the developing cell plate. In order to understand the functional implications of this novel arrangement, especially in onion, which has hitherto been regarded as a model system in which to study plant cell division, we investigated how widespread the phenomenon was in selected monocots and dicots. During monocot cytokinesis, peroxisomes lacked aggregation in some taxa, notably grasses, and underwent partial aggregation in other taxa. However, complete aggregation of a cell's entire complement of peroxisomes was restricted to the genus Allium. Although peroxisomal aggregation has been suggested to function in the formation of the cell plate, the distribution of partial and complete aggregation in monocots did not match known differences in primary cell wall biochemistry. No aggregation was seen during cytokinesis in dicots. Through quantification of peroxisome distribution in cells whose actin microfilaments were disrupted with either latrunculin or cytochalasin, we demonstrated that peroxisomal aggregation is a microfilament-dependent process in Allium and the closely related plant Tristagma, which shows only partial aggregation. We speculate whether analysis of the peroxisomal proteome might reveal novel function(s) for aggregated peroxisomes during cytokinesis

KW - Open access version available

KW - Actin microfilaments

KW - Allium

KW - Cell division

KW - Microtubules

KW - Peroxisomes

KW - Phragmoplast

U2 - 10.1086/523965

DO - 10.1086/523965

M3 - Article

VL - 169

SP - 241

EP - 252

JO - Botanical Gazette

JF - Botanical Gazette

SN - 1058-5893

IS - 2

ER -