PGRMC1 effects on metabolism, genomic mutation and CpG methylation imply crucial roles in animal biology and disease

Bashar M Thejer, Partho P Adhikary, Sarah L Teakel, Johnny Fang, Paul A Weston, Saliya Gurusinghe, Ayad G Anwer, Martin Gosnell, Jalal A Jazayeri, Marina Ludescher, Lesley-Ann Gray, Michael Pawlak, Robyn H Wallace, Sameer D Pant, Marie Wong, Tamas Fischer, Elizabeth J New, Tanja N Fehm, Hans Neubauer, Ewa M GoldysJane C Quinn, Leslie A Weston, Michael A Cahill

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Progesterone receptor membrane component 1 (PGRMC1) is often elevated in cancers, and exists in alternative states of phosphorylation. A motif centered on PGRMC1 Y180 was evolutionarily acquired concurrently with the embryological gastrulation organizer that orchestrates vertebrate tissue differentiation. Results:
Here, we show that mutagenic manipulation of PGRMC1 phosphorylation alters cell metabolism, genomic stability, and CpG methylation. Each of several mutants elicited distinct patterns of genomic CpG methylation. Mutation of S57A/Y180/S181A led to increased net hypermethylation, reminiscent of embryonic stem cells. Pathways enrichment analysis suggested modulation of processes related to animal cell differentiation status and tissue identity, as well as cell cycle control and ATM/ATR DNA damage repair regulation. We detected different genomic mutation rates in culture. Conclusions: A companion manuscript shows that these cell states dramatically affect protein abundances, cell and mitochondrial morphology, and glycolytic metabolism. We propose that PGRMC1 phosphorylation status modulates cellular plasticity mechanisms relevant to early embryological tissue differentiation.
Original languageEnglish
Article number26
Pages (from-to)1-19
Number of pages19
JournalBMC Molecular and Cell Biology
Volume21
DOIs
Publication statusPublished - 15 Apr 2020

Fingerprint

Dive into the research topics of 'PGRMC1 effects on metabolism, genomic mutation and CpG methylation imply crucial roles in animal biology and disease'. Together they form a unique fingerprint.

Cite this