Pharmacokinetics of Equine Medications ' Hydrocortisone: Method validation and preliminary results.

J.H. Vine, L.L. Glowacki, Fiona Schneiders, Glenys Noble

Research output: Book chapter/Published conference paperConference paperpeer-review


A consortium consisting of the four Australian racing laboratories, three Australian universities and supported by the Rural Industries Research and Development Corporation has been formed to carry out pharmacokinetic, metabolic and excretion studies on commonly used veterinary medications with the aim of providing reliable detection guidelines in racing horses. Studies are carried out in 10-12 horses for each drug to ensure that representative data are obtained.Hydrocortisone is a naturally-occurring corticosteroid which is commonly used in veterinary medicine as an anti-inflammatory agent. It is usually administered by intravenous or intramuscular injection in the form of the sodium salt of the hemisuccinate ester.In this study hydrocortisone (1g hydrocortisone sodium succinate) was administered by intravenous injection to 10 horses. Blood and urine samples were collected for a week prior to dosing so that normal baseline levels could be determined then for 7 days after dosing.Analytical method validation studies have been carried out for hydrocortisone hemisuccinate, hydrocortisone, its metabolic precursors and its metabolites with and without enzyme hydrolysis, using liquid/liquid and solid phase extraction methods with d4-hydrocortisone and triamcinolone acetonide as internal standards. Analysis was carried out by LCMSMS. Precision and accuracy were determined by intra- and inter-day analysis of control materials.This paper will also report on the results of preliminary studies to determine which target analytes should be used in the planned pharmacokinetic studies and on whether there may be alternative approaches to the use of the hydrocortisone threshold concentration for drug control purposes in racing.
Original languageEnglish
Title of host publicationICRAV 2008, 17th International Conference of Racing Analysts and Veterinarians.
EditorsN Yazicioglu N Yazicioglu
Place of PublicationNewmarket, UK
PublisherR and W Publications
ISBN (Electronic)9781899772155
Publication statusPublished - 2008
EventInternational Conference of Racing Analysts and Veterinarians - Antalya, Turkey, Turkey
Duration: 11 Oct 200817 Oct 2008


ConferenceInternational Conference of Racing Analysts and Veterinarians


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