A consortium consisting of the four Australian racing laboratories, three Australian universities and supported by the Rural Industries Research and Development Corporation has been formed to carry out pharmacokinetic, metabolic and excretion studies on commonly used veterinary medications with the aim of providing reliable detection guidelines in racing horses. Studies are carried out in 10-12 horses for each drug to ensure that representative data are obtained.Hydrocortisone is a naturally-occurring corticosteroid which is commonly used in veterinary medicine as an anti-inflammatory agent. It is usually administered by intravenous or intramuscular injection in the form of the sodium salt of the hemisuccinate ester.In this study hydrocortisone (1g hydrocortisone sodium succinate) was administered by intravenous injection to 10 horses. Blood and urine samples were collected for a week prior to dosing so that normal baseline levels could be determined then for 7 days after dosing.Analytical method validation studies have been carried out for hydrocortisone hemisuccinate, hydrocortisone, its metabolic precursors and its metabolites with and without enzyme hydrolysis, using liquid/liquid and solid phase extraction methods with d4-hydrocortisone and triamcinolone acetonide as internal standards. Analysis was carried out by LCMSMS. Precision and accuracy were determined by intra- and inter-day analysis of control materials.This paper will also report on the results of preliminary studies to determine which target analytes should be used in the planned pharmacokinetic studies and on whether there may be alternative approaches to the use of the hydrocortisone threshold concentration for drug control purposes in racing.
|Title of host publication||ICRAV 2008, 17th International Conference of Racing Analysts and Veterinarians.|
|Editors||N Yazicioglu N Yazicioglu|
|Place of Publication||Newmarket, UK|
|Publisher||R and W Publications|
|Publication status||Published - 2008|
|Event||International Conference of Racing Analysts and Veterinarians - Antalya, Turkey, Turkey|
Duration: 11 Oct 2008 → 17 Oct 2008
|Conference||International Conference of Racing Analysts and Veterinarians|
|Period||11/10/08 → 17/10/08|
Vine, J. H., Glowacki, L. L., Schneiders, F., & Noble, G. (2008). Pharmacokinetics of Equine Medications ' Hydrocortisone: Method validation and preliminary results. In N. Y. N. Yazicioglu (Ed.), ICRAV 2008, 17th International Conference of Racing Analysts and Veterinarians. (pp. tba). R and W Publications.