Phosphorus remobilization from rice flag leaves during grain filling: An RNA-seq study

Kwanho Jeong, Abdul Baten, Daniel L.E. Waters, Omar Pantoja, Cecile C. Julia, Matthias Wissuwa, Sigrid Heuer, Tobias Kretzschmar, Terry J. Rose

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21 Citations (Scopus)
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Abstract

The physiology and molecular regulation of phosphorus (P) remobilization from vegetative tissues to grains during grain filling is poorly understood, despite the pivotal role it plays in the global P cycle. To test the hypothesis that a subset of genes involved in the P starvation response are involved in remobilization of P from flag leaves to developing grains, we conducted an RNA-seq analysis of rice flag leaves during the preremobilization phase (6 DAA) and when the leaves were acting as a P source (15 DAA). Several genes that respond to phosphate starvation, including three purple acid phosphatases (OsPAP3, OsPAP9b and OsPAP10a), were significantly up-regulated at 15 DAA, consistent with a role in remobilization of P from flag leaves during grain filling. A number of genes that have not been implicated in the phosphate starvation response, OsPAP26, SPX-MFS1 (a putative P transporter) and SPX-MFS2, also showed expression profiles consistent with involvement in P remobilization from senescing flag leaves. Metabolic pathway analysis using the KEGG system suggested plastid membrane lipid synthesis is a critical process during the P remobilization phase. In particular, the up-regulation of OsPLDz2 and OsSQD2 at 15 DAA suggested phospholipids were being degraded and replaced by other lipids to enable continued cellular function while liberating P for export to developing grains. Three genes associated with RNA degradation that have not previously been implicated in the P starvation response also showed expression profiles consistent with a role in P mobilization from senescing flag leaves.

Original languageEnglish
Pages (from-to)15-26
Number of pages12
JournalPlant Biotechnology Journal
Volume15
Issue number1
Early online dateMay 2016
DOIs
Publication statusPublished - 01 Jan 2017

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    Jeong, K., Baten, A., Waters, D. L. E., Pantoja, O., Julia, C. C., Wissuwa, M., Heuer, S., Kretzschmar, T., & Rose, T. J. (2017). Phosphorus remobilization from rice flag leaves during grain filling: An RNA-seq study. Plant Biotechnology Journal, 15(1), 15-26. https://doi.org/10.1111/pbi.12586