Pigmented rice-derived phenolic compounds reduce biomarkers of oxidative stress and inflammation in human umbilical vein endothelial cells

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Abstract

Scope: Endothelial dysfunction pathogenesis is significantly associated with increased oxidative stress (OS) and inflammation. Rice-derived phenolic compounds have been demonstrated to have antioxidant and anti-inflammatory potential. This study aims to determine if phenolic extracts (PE) from pigmented rice varieties (Purple, Yunlu29–red, and Reiziq–brown) at varying concentrations modulate biomarkers of OS and inflammation in human umbilical vein endothelial cells under induced OS conditions. Methods and results: 2,7-dichlorofluorescein diacetate and superoxide dismutase-1 (SOD-1) enzyme-linked immuno-sorbent assay quantification demonstrate that Purple PE significantly decreases reactive oxygen species and increases SOD-1 by 27% and 226%, respectively. Yunlu29 PE (50 µg mL−1) is the most effective in reducing (p < 0.0001) interleukin-8 (61%) and interleukin-6 (57.2%). Yunlu29 (50 µg mL−1) reduces intracellular-adhesion molecule-1 (p < 0.0001) expression by 34%, followed by Reiziq (31.9%) and Purple (30.2%). Flow cytometric analysis demonstrates that vascular cell-adhesion molecule-1 expression is reduced (p < 0.0001) by 53.5% by Yunlu29 followed by Purple (46.8%) and Reiziq (46.7%). Yunlu29 is the most effective in reducing nuclear factor kappa-B expression by 50.1%, followed by purple (48.8%) and Reiziq (38.6%). Conclusion: This study indicates that colored rice PE may potentially target OS and inflammatory pathways associated with the pathogenesis of endothelial dysfunction. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Original languageEnglish
Article number1800840
Pages (from-to)1-8
Number of pages8
JournalMolecular Nutrition and Food Research
Volume62
Issue number24
Early online date01 Nov 2018
DOIs
Publication statusPublished - 20 Dec 2018

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Human Umbilical Vein Endothelial Cells
phenolic compounds
biomarkers
Oxidative Stress
oxidative stress
inflammation
Biomarkers
Inflammation
rice
extracts
superoxide dismutase
pathogenesis
Vascular Cell Adhesion Molecule-1
NF-kappa B
interleukin-8
anti-inflammatory activity
Interleukin-8
cell adhesion
blood vessels
interleukin-6

Cite this

@article{85900c2b046c447bb05b4e5e230b2cd1,
title = "Pigmented rice-derived phenolic compounds reduce biomarkers of oxidative stress and inflammation in human umbilical vein endothelial cells",
abstract = "Scope: Endothelial dysfunction pathogenesis is significantly associated with increased oxidative stress (OS) and inflammation. Rice-derived phenolic compounds have been demonstrated to have antioxidant and anti-inflammatory potential. This study aims to determine if phenolic extracts (PE) from pigmented rice varieties (Purple, Yunlu29–red, and Reiziq–brown) at varying concentrations modulate biomarkers of OS and inflammation in human umbilical vein endothelial cells under induced OS conditions. Methods and results: 2,7-dichlorofluorescein diacetate and superoxide dismutase-1 (SOD-1) enzyme-linked immuno-sorbent assay quantification demonstrate that Purple PE significantly decreases reactive oxygen species and increases SOD-1 by 27{\%} and 226{\%}, respectively. Yunlu29 PE (50 µg mL−1) is the most effective in reducing (p < 0.0001) interleukin-8 (61{\%}) and interleukin-6 (57.2{\%}). Yunlu29 (50 µg mL−1) reduces intracellular-adhesion molecule-1 (p < 0.0001) expression by 34{\%}, followed by Reiziq (31.9{\%}) and Purple (30.2{\%}). Flow cytometric analysis demonstrates that vascular cell-adhesion molecule-1 expression is reduced (p < 0.0001) by 53.5{\%} by Yunlu29 followed by Purple (46.8{\%}) and Reiziq (46.7{\%}). Yunlu29 is the most effective in reducing nuclear factor kappa-B expression by 50.1{\%}, followed by purple (48.8{\%}) and Reiziq (38.6{\%}). Conclusion: This study indicates that colored rice PE may potentially target OS and inflammatory pathways associated with the pathogenesis of endothelial dysfunction. {\circledC} 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",
author = "Esther Callcott and Christopher Blanchard and Prakash Oli and Abishek Santhakumar",
year = "2018",
month = "12",
day = "20",
doi = "10.1002/mnfr.201800840",
language = "English",
volume = "62",
pages = "1--8",
journal = "Molecular Nutrition and Food Research",
issn = "1613-4125",
publisher = "Wiley-VCH Verlag",
number = "24",

}

TY - JOUR

T1 - Pigmented rice-derived phenolic compounds reduce biomarkers of oxidative stress and inflammation in human umbilical vein endothelial cells

AU - Callcott, Esther

AU - Blanchard, Christopher

AU - Oli, Prakash

AU - Santhakumar, Abishek

PY - 2018/12/20

Y1 - 2018/12/20

N2 - Scope: Endothelial dysfunction pathogenesis is significantly associated with increased oxidative stress (OS) and inflammation. Rice-derived phenolic compounds have been demonstrated to have antioxidant and anti-inflammatory potential. This study aims to determine if phenolic extracts (PE) from pigmented rice varieties (Purple, Yunlu29–red, and Reiziq–brown) at varying concentrations modulate biomarkers of OS and inflammation in human umbilical vein endothelial cells under induced OS conditions. Methods and results: 2,7-dichlorofluorescein diacetate and superoxide dismutase-1 (SOD-1) enzyme-linked immuno-sorbent assay quantification demonstrate that Purple PE significantly decreases reactive oxygen species and increases SOD-1 by 27% and 226%, respectively. Yunlu29 PE (50 µg mL−1) is the most effective in reducing (p < 0.0001) interleukin-8 (61%) and interleukin-6 (57.2%). Yunlu29 (50 µg mL−1) reduces intracellular-adhesion molecule-1 (p < 0.0001) expression by 34%, followed by Reiziq (31.9%) and Purple (30.2%). Flow cytometric analysis demonstrates that vascular cell-adhesion molecule-1 expression is reduced (p < 0.0001) by 53.5% by Yunlu29 followed by Purple (46.8%) and Reiziq (46.7%). Yunlu29 is the most effective in reducing nuclear factor kappa-B expression by 50.1%, followed by purple (48.8%) and Reiziq (38.6%). Conclusion: This study indicates that colored rice PE may potentially target OS and inflammatory pathways associated with the pathogenesis of endothelial dysfunction. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

AB - Scope: Endothelial dysfunction pathogenesis is significantly associated with increased oxidative stress (OS) and inflammation. Rice-derived phenolic compounds have been demonstrated to have antioxidant and anti-inflammatory potential. This study aims to determine if phenolic extracts (PE) from pigmented rice varieties (Purple, Yunlu29–red, and Reiziq–brown) at varying concentrations modulate biomarkers of OS and inflammation in human umbilical vein endothelial cells under induced OS conditions. Methods and results: 2,7-dichlorofluorescein diacetate and superoxide dismutase-1 (SOD-1) enzyme-linked immuno-sorbent assay quantification demonstrate that Purple PE significantly decreases reactive oxygen species and increases SOD-1 by 27% and 226%, respectively. Yunlu29 PE (50 µg mL−1) is the most effective in reducing (p < 0.0001) interleukin-8 (61%) and interleukin-6 (57.2%). Yunlu29 (50 µg mL−1) reduces intracellular-adhesion molecule-1 (p < 0.0001) expression by 34%, followed by Reiziq (31.9%) and Purple (30.2%). Flow cytometric analysis demonstrates that vascular cell-adhesion molecule-1 expression is reduced (p < 0.0001) by 53.5% by Yunlu29 followed by Purple (46.8%) and Reiziq (46.7%). Yunlu29 is the most effective in reducing nuclear factor kappa-B expression by 50.1%, followed by purple (48.8%) and Reiziq (38.6%). Conclusion: This study indicates that colored rice PE may potentially target OS and inflammatory pathways associated with the pathogenesis of endothelial dysfunction. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

U2 - 10.1002/mnfr.201800840

DO - 10.1002/mnfr.201800840

M3 - Article

C2 - 30382609

VL - 62

SP - 1

EP - 8

JO - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

SN - 1613-4125

IS - 24

M1 - 1800840

ER -