TY - JOUR
T1 - Poly(A) Extensions of miRNAs for Amplification-Free Electrochemical Detection on Screen-Printed Gold Electrodes
AU - Koo, Kevin M.
AU - Carrascosa, Laura G.
AU - Shiddiky, Muhammad J.A.
AU - Trau, Matt
N1 - Funding Information:
This work was supported by the NHMRC CDF (Grant APP1088966), ARC DP (Grant DP140104006), and UQ Postdoctoral Research Fellowship (Grant 2012001456). Although not directly funding the research work in this paper, we acknowledge funding received by our laboratory from the National Breast Cancer Foundation of Australia (Grant CG-12-07). This grant has significantly contributed to the environment to stimulate the research described here.
Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/2/16
Y1 - 2016/2/16
N2 - Current amplification-based microRNA (miRNA) detection approaches are limited by the small sizes of miRNAs as well as amplification bias/artifacts. Herein, we report on an amplification-free miRNA assay based on elevated affinity interaction between polyadenylated miRNA and bare gold electrode. The poly(A) extension on the 3' ends of magnetically isolated miRNA targets facilitated high adsorption efficiency onto gold electrode surfaces for electrochemical detection without any cumbersome electrode surface functionalization procedures. The assay showed excellent detection sensitivity (10 fM) and specificity and was demonstrated for quantitative miR-107 detection in human cancer cell lines and clinical urine samples. We believe our assay could be useful as an amplification-free alternative for miRNA detection.
AB - Current amplification-based microRNA (miRNA) detection approaches are limited by the small sizes of miRNAs as well as amplification bias/artifacts. Herein, we report on an amplification-free miRNA assay based on elevated affinity interaction between polyadenylated miRNA and bare gold electrode. The poly(A) extension on the 3' ends of magnetically isolated miRNA targets facilitated high adsorption efficiency onto gold electrode surfaces for electrochemical detection without any cumbersome electrode surface functionalization procedures. The assay showed excellent detection sensitivity (10 fM) and specificity and was demonstrated for quantitative miR-107 detection in human cancer cell lines and clinical urine samples. We believe our assay could be useful as an amplification-free alternative for miRNA detection.
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U2 - 10.1021/acs.analchem.5b04795
DO - 10.1021/acs.analchem.5b04795
M3 - Article
C2 - 26814930
AN - SCOPUS:84958291845
SN - 0003-2700
VL - 88
SP - 2000
EP - 2005
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 4
ER -
