Polypyrrole-based potentiometric biosensor for urea. Part 2. Analytical optimisation

S. B. Adeloju, S. J. Shaw, G. G. Wallace

Research output: Contribution to journalArticlepeer-review

75 Citations (Scopus)


A novel potentiometric biosensor for urea, based on the entrapment of urease into a polypyrrole film, has been developed via galavanostatic film formation. The electrochemical polymerization was achieved by the application of a current density of 0.5 mA cm-2 to a working platinum disc electrode for 3 min in a solution containing 0.5 M pyrrole and 4000 μ ml-1 urease. The optimum conditions for the reliable performance of this sensor were 1 mM phosphate buffer at pH 7.0 and 35°C. A Nerstein response was observed for increasing concentration of urea in the linear range of 0.5 to 100mM. Trace concentrations of Hg2+ and K+ were both found to inhibit the response of the biosensor.

Original languageEnglish
Pages (from-to)621-627
Number of pages7
JournalAnalytica Chimica Acta
Issue number3
Publication statusPublished - 24 Sep 1993


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