TY - JOUR
T1 - Prediction of intramuscular fat content and major fatty acid groups of lamb M. longissimus lumborum using Raman spectroscopy
AU - Fowler, Stephanie M.
AU - Ponnampalam, Eric N.
AU - Schmidt, Heinar
AU - Wynn, Peter
AU - Hopkins, David L.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - A hand held Raman spectroscopic device was used to predict intramuscular fat (IMF) levels and the major fatty acid (FA) groups of fresh intact ovine M. longissimus lumborum (LL). IMF levels were determined using the Soxhlet method, while FA analysis was conducted using a rapid (KOH in water, methanol and sulphuric acid in water) extraction procedure. IMF levels and FA values were regressed against Raman spectra using partial least squares regression and against each other using linear regression. The results indicate that there is potential to predict PUFA (R 2 =0.93) and MUFA (R 2 =0.54) as well as SFA values that had been adjusted for IMF content (R 2 =0.54). However, this potential was significantly reduced when correlations between predicted and observed values were determined by cross validation (R 2
cv =0.21-0.00). Overall, the prediction of major FA groups using Raman spectra was more precise (relative reductions in error of 0.3-40.8%) compared to the null models.
AB - A hand held Raman spectroscopic device was used to predict intramuscular fat (IMF) levels and the major fatty acid (FA) groups of fresh intact ovine M. longissimus lumborum (LL). IMF levels were determined using the Soxhlet method, while FA analysis was conducted using a rapid (KOH in water, methanol and sulphuric acid in water) extraction procedure. IMF levels and FA values were regressed against Raman spectra using partial least squares regression and against each other using linear regression. The results indicate that there is potential to predict PUFA (R 2 =0.93) and MUFA (R 2 =0.54) as well as SFA values that had been adjusted for IMF content (R 2 =0.54). However, this potential was significantly reduced when correlations between predicted and observed values were determined by cross validation (R 2
cv =0.21-0.00). Overall, the prediction of major FA groups using Raman spectra was more precise (relative reductions in error of 0.3-40.8%) compared to the null models.
KW - Lamb
KW - Major fatty acid groups
KW - Meat quality assessment
KW - Raman spectroscopy
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U2 - 10.1016/j.meatsci.2015.06.016
DO - 10.1016/j.meatsci.2015.06.016
M3 - Article
C2 - 26188359
AN - SCOPUS:84941564693
VL - 110
SP - 70
EP - 75
JO - Meat Science
JF - Meat Science
SN - 0309-1740
ER -