Prediction of starch reserves in intact and ground grapevine cane wood tissues using near‐infrared reflectance spectroscopy

Joanna E. Jones, Alieta Eyles, Caroline Claye, Thomas Rodemann, Robert Dambergs, Dugald Close

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

BACKGROUND: Near-infrared reflectance spectroscopy (NIRS) technology can be a powerful analytical technique for the assessment of plant starch, but generally samples need to be freeze-dried and ground. This study investigated the feasibility of using NIRS technology to quantify starch concentration in ground and intact grapevine cane wood samples (with or without the bark layer). A partial least squares regression was used on the sample spectral data and was compared against starch analysis using a conventional wet chemistry method.
RESULTS: Accurate calibration models were obtained for the ground cane wood samples (n = 220), one based on 17 factors
(R2 = 0.88, root mean square error of validation (RMSEV) of 0.73 mg g−1) and the other based on 10 factors (R2 = 0.85, RMSEV of 0.80 mg g−1). In contrast, the prediction of starch within intact cane wood samples was very low (R2 = 0.19). Removal of the cane bark tissues did not substantially improve the accuracy of the model (R2 = 0.34). Despite these poor correlations and low ratio of prediction to deviation values of 1.08–1.24, the root mean square error of cross-validation (RMSECV) values were
0.75–0.86 mg g−1, indicating good predictability of the model.
CONCLUSIONS: As indicated by low RMSECV values, NIRS technology has the potential to monitor grapevine starch reserves in intact cane wood samples.
Original languageEnglish
Pages (from-to)2418-2424
Number of pages7
JournalJournal of the Science of Food and Agriculture
Volume100
Issue number6
Early online date09 Jan 2020
DOIs
Publication statusPublished - Apr 2020

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