Prevalence and spectrum of Johne’s disease lesions in cattle slaughtered at two abattoirs in Kampala, Uganda

Poster 131. 56th AAVLD/117th USAHA Annual Meeting Proceedings

Julius Boniface Okuni, Manfred Reinacher, Panayiotis Loukopoulos, Lonzy Ojok

Research output: Other contribution to conferencePoster

3 Citations (Scopus)
138 Downloads (Pure)

Abstract

This study was conducted to determine the prevalence and characteristics of Johne’s disease (JD) lesions in Ugandan cattle slaughtered at two of the main abattoirs in Kampala. JD, or paratuberculosis, is a chronic wasting
disease that affects a wide range of domestic and wild animals and is caused by Mycobacterium avium subspecies paratuberculosis (MAP), an acid-fast bacterium. It has serious negative impact on the productivity of the affected
animals. The control and eradication of the disease is constrained by the lack of early diagnosis tools. Identification of the affected animals can be based on clinical signs, demonstration of the organism in feces and tissues of affected
animals using various bacteriological and molecular methods; serological testing in live animals, and gross and microscopic lesions. In countries where JD has never been reported or has very low prevalence, facilities for culture
and molecular diagnosis of the disease might not be in existence. Under such circumstances, pathological diagnosis is very important. Although gross lesions can lead to a correct diagnosis of the disease in advanced stages, in some
cases, even animals with advanced disease may lack such lesions. Moreover, in early stages of the disease, the lesions may be so subtle as to escape recognition. Ileocecal junction and the associated lymph nodes of 1,022 cattle
were examined for gross and microscopic lesions, followed by Ziehl Neelsen staining of the tissues bearing lesions. Confirmation of MAP infection was done in some of the tissues using culture and IS900 PCR. The lesions were then
described, characterised and tabulated. Characteristic JD granulomas were found in 4.7% of the samples examined, derived from Zebu, Ankole longhorn, Friesian breeds of cattle and their crosses. Lesions were found both in the
lymph nodes and ileocecal junction mucosa. The lesions tended to be more severe in the lymph node than in the mucosa. There were also some unique and atypical lesions found in association with JD granulomas. The diagnostic
value of various gross lesions and criteria of lesion classifications and diagnosis are revisited and discussed based on the findings of this study. The prevalence of JD lesions among slaughtered cattle in Kampala’s two abattoirs
indicates that the disease is well established in the cattle population in the country. The diverse manifestations in lesions of JD, particularly early, focal or mild lesions, need to be considered when making histological diagnosis in
tissues where the disease is suspected.
Original languageEnglish
Pages192-192
Number of pages1
Publication statusPublished - Oct 2013
Event56th American Association of Veterinary Laboratory Diagnosticians (AAVLD)/117th United States Animal Hospital Association (USAHA) Annual Meeting - Town and Country Resort & Convention Center , San Diego, United States
Duration: 16 Oct 201322 Oct 2013
http://www.aavld.org/index.php?option=com_content&view=article&id=315:program-guide-annual-meeting-2013

Conference

Conference56th American Association of Veterinary Laboratory Diagnosticians (AAVLD)/117th United States Animal Hospital Association (USAHA) Annual Meeting
CountryUnited States
CitySan Diego
Period16/10/1322/10/13
Internet address

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Paratuberculosis
Posters
Abattoirs
Uganda
Mycobacterium avium subsp. paratuberculosis
Granuloma
Mucous Membrane
Lymph Nodes
Disease Eradication
Wild Animals
Domestic Animals
Feces
Early Diagnosis
Staining and Labeling
Bacteria
Polymerase Chain Reaction
Acids

Cite this

Okuni, J. B., Reinacher, M., Loukopoulos, P., & Ojok, L. (2013). Prevalence and spectrum of Johne’s disease lesions in cattle slaughtered at two abattoirs in Kampala, Uganda: Poster 131. 56th AAVLD/117th USAHA Annual Meeting Proceedings. 192-192. Poster session presented at 56th American Association of Veterinary Laboratory Diagnosticians (AAVLD)/117th United States Animal Hospital Association (USAHA) Annual Meeting, San Diego, United States.
Okuni, Julius Boniface ; Reinacher, Manfred ; Loukopoulos, Panayiotis ; Ojok, Lonzy. / Prevalence and spectrum of Johne’s disease lesions in cattle slaughtered at two abattoirs in Kampala, Uganda : Poster 131. 56th AAVLD/117th USAHA Annual Meeting Proceedings. Poster session presented at 56th American Association of Veterinary Laboratory Diagnosticians (AAVLD)/117th United States Animal Hospital Association (USAHA) Annual Meeting, San Diego, United States.1 p.
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abstract = "This study was conducted to determine the prevalence and characteristics of Johne’s disease (JD) lesions in Ugandan cattle slaughtered at two of the main abattoirs in Kampala. JD, or paratuberculosis, is a chronic wastingdisease that affects a wide range of domestic and wild animals and is caused by Mycobacterium avium subspecies paratuberculosis (MAP), an acid-fast bacterium. It has serious negative impact on the productivity of the affectedanimals. The control and eradication of the disease is constrained by the lack of early diagnosis tools. Identification of the affected animals can be based on clinical signs, demonstration of the organism in feces and tissues of affectedanimals using various bacteriological and molecular methods; serological testing in live animals, and gross and microscopic lesions. In countries where JD has never been reported or has very low prevalence, facilities for cultureand molecular diagnosis of the disease might not be in existence. Under such circumstances, pathological diagnosis is very important. Although gross lesions can lead to a correct diagnosis of the disease in advanced stages, in somecases, even animals with advanced disease may lack such lesions. Moreover, in early stages of the disease, the lesions may be so subtle as to escape recognition. Ileocecal junction and the associated lymph nodes of 1,022 cattlewere examined for gross and microscopic lesions, followed by Ziehl Neelsen staining of the tissues bearing lesions. Confirmation of MAP infection was done in some of the tissues using culture and IS900 PCR. The lesions were thendescribed, characterised and tabulated. Characteristic JD granulomas were found in 4.7{\%} of the samples examined, derived from Zebu, Ankole longhorn, Friesian breeds of cattle and their crosses. Lesions were found both in thelymph nodes and ileocecal junction mucosa. The lesions tended to be more severe in the lymph node than in the mucosa. There were also some unique and atypical lesions found in association with JD granulomas. The diagnosticvalue of various gross lesions and criteria of lesion classifications and diagnosis are revisited and discussed based on the findings of this study. The prevalence of JD lesions among slaughtered cattle in Kampala’s two abattoirsindicates that the disease is well established in the cattle population in the country. The diverse manifestations in lesions of JD, particularly early, focal or mild lesions, need to be considered when making histological diagnosis intissues where the disease is suspected.",
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Okuni, JB, Reinacher, M, Loukopoulos, P & Ojok, L 2013, 'Prevalence and spectrum of Johne’s disease lesions in cattle slaughtered at two abattoirs in Kampala, Uganda: Poster 131. 56th AAVLD/117th USAHA Annual Meeting Proceedings' 56th American Association of Veterinary Laboratory Diagnosticians (AAVLD)/117th United States Animal Hospital Association (USAHA) Annual Meeting, San Diego, United States, 16/10/13 - 22/10/13, pp. 192-192.

Prevalence and spectrum of Johne’s disease lesions in cattle slaughtered at two abattoirs in Kampala, Uganda : Poster 131. 56th AAVLD/117th USAHA Annual Meeting Proceedings. / Okuni, Julius Boniface; Reinacher, Manfred; Loukopoulos, Panayiotis; Ojok, Lonzy.

2013. 192-192 Poster session presented at 56th American Association of Veterinary Laboratory Diagnosticians (AAVLD)/117th United States Animal Hospital Association (USAHA) Annual Meeting, San Diego, United States.

Research output: Other contribution to conferencePoster

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T1 - Prevalence and spectrum of Johne’s disease lesions in cattle slaughtered at two abattoirs in Kampala, Uganda

T2 - Poster 131. 56th AAVLD/117th USAHA Annual Meeting Proceedings

AU - Okuni, Julius Boniface

AU - Reinacher, Manfred

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AU - Ojok, Lonzy

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N2 - This study was conducted to determine the prevalence and characteristics of Johne’s disease (JD) lesions in Ugandan cattle slaughtered at two of the main abattoirs in Kampala. JD, or paratuberculosis, is a chronic wastingdisease that affects a wide range of domestic and wild animals and is caused by Mycobacterium avium subspecies paratuberculosis (MAP), an acid-fast bacterium. It has serious negative impact on the productivity of the affectedanimals. The control and eradication of the disease is constrained by the lack of early diagnosis tools. Identification of the affected animals can be based on clinical signs, demonstration of the organism in feces and tissues of affectedanimals using various bacteriological and molecular methods; serological testing in live animals, and gross and microscopic lesions. In countries where JD has never been reported or has very low prevalence, facilities for cultureand molecular diagnosis of the disease might not be in existence. Under such circumstances, pathological diagnosis is very important. Although gross lesions can lead to a correct diagnosis of the disease in advanced stages, in somecases, even animals with advanced disease may lack such lesions. Moreover, in early stages of the disease, the lesions may be so subtle as to escape recognition. Ileocecal junction and the associated lymph nodes of 1,022 cattlewere examined for gross and microscopic lesions, followed by Ziehl Neelsen staining of the tissues bearing lesions. Confirmation of MAP infection was done in some of the tissues using culture and IS900 PCR. The lesions were thendescribed, characterised and tabulated. Characteristic JD granulomas were found in 4.7% of the samples examined, derived from Zebu, Ankole longhorn, Friesian breeds of cattle and their crosses. Lesions were found both in thelymph nodes and ileocecal junction mucosa. The lesions tended to be more severe in the lymph node than in the mucosa. There were also some unique and atypical lesions found in association with JD granulomas. The diagnosticvalue of various gross lesions and criteria of lesion classifications and diagnosis are revisited and discussed based on the findings of this study. The prevalence of JD lesions among slaughtered cattle in Kampala’s two abattoirsindicates that the disease is well established in the cattle population in the country. The diverse manifestations in lesions of JD, particularly early, focal or mild lesions, need to be considered when making histological diagnosis intissues where the disease is suspected.

AB - This study was conducted to determine the prevalence and characteristics of Johne’s disease (JD) lesions in Ugandan cattle slaughtered at two of the main abattoirs in Kampala. JD, or paratuberculosis, is a chronic wastingdisease that affects a wide range of domestic and wild animals and is caused by Mycobacterium avium subspecies paratuberculosis (MAP), an acid-fast bacterium. It has serious negative impact on the productivity of the affectedanimals. The control and eradication of the disease is constrained by the lack of early diagnosis tools. Identification of the affected animals can be based on clinical signs, demonstration of the organism in feces and tissues of affectedanimals using various bacteriological and molecular methods; serological testing in live animals, and gross and microscopic lesions. In countries where JD has never been reported or has very low prevalence, facilities for cultureand molecular diagnosis of the disease might not be in existence. Under such circumstances, pathological diagnosis is very important. Although gross lesions can lead to a correct diagnosis of the disease in advanced stages, in somecases, even animals with advanced disease may lack such lesions. Moreover, in early stages of the disease, the lesions may be so subtle as to escape recognition. Ileocecal junction and the associated lymph nodes of 1,022 cattlewere examined for gross and microscopic lesions, followed by Ziehl Neelsen staining of the tissues bearing lesions. Confirmation of MAP infection was done in some of the tissues using culture and IS900 PCR. The lesions were thendescribed, characterised and tabulated. Characteristic JD granulomas were found in 4.7% of the samples examined, derived from Zebu, Ankole longhorn, Friesian breeds of cattle and their crosses. Lesions were found both in thelymph nodes and ileocecal junction mucosa. The lesions tended to be more severe in the lymph node than in the mucosa. There were also some unique and atypical lesions found in association with JD granulomas. The diagnosticvalue of various gross lesions and criteria of lesion classifications and diagnosis are revisited and discussed based on the findings of this study. The prevalence of JD lesions among slaughtered cattle in Kampala’s two abattoirsindicates that the disease is well established in the cattle population in the country. The diverse manifestations in lesions of JD, particularly early, focal or mild lesions, need to be considered when making histological diagnosis intissues where the disease is suspected.

KW - Veterinary pathology

KW - Paratuberculosis

KW - Cattle Diseases

M3 - Poster

SP - 192

EP - 192

ER -

Okuni JB, Reinacher M, Loukopoulos P, Ojok L. Prevalence and spectrum of Johne’s disease lesions in cattle slaughtered at two abattoirs in Kampala, Uganda: Poster 131. 56th AAVLD/117th USAHA Annual Meeting Proceedings. 2013. Poster session presented at 56th American Association of Veterinary Laboratory Diagnosticians (AAVLD)/117th United States Animal Hospital Association (USAHA) Annual Meeting, San Diego, United States.