Prevalence of Mycoplasma bovis in a southern NSW feedlot during high and low risk periods for bovine respiratory disease

Rebecca Barnewall; Ian Marsh; Narelle Sales; L Stroud; Thom Williams; Michael Campbell; Paul Cusack; Jane Quinn

Abstract

Bovine respiratory disease (BRD) is a multifactorial disorder in which the proliferation of bacterial pathogens may be influenced by pre-exposure to viral pathogens and behavioural and environmental stressors. The role of Mycoplasma bovis in the pathogenicity of BRD, as a non-commensal bacterium, is poorly understood and its recalcitrant nature complicates the detection in clinical samples. To overcome the limitations of conventional culture, we have utilised an absolute quantitative PCR approach (Kishimoto et al. 2017) to improve the detection of M. bovis in feedlot steers. To better understand the prevalence of this organism in the Australian feedlot system, we compared induction to hospital pen animals using qPCR and compared two DNA extraction techniques.

Original language	English
Pages	cxci
Number of pages	1
Publication status	Published - Jan 2021
Event	33rd Biennial Conference of the Australian Association of Animal Sciences - Esplanade Hotel Fremantle by Rydges, Perth, Australia Duration: 01 Feb 2021 → 03 Feb 2021 Conference number: 33 http://www.animalscienceconference.com.au/ https://www.publish.csiro.au/AN/ANv61n3abs (Abstracts)

Conference

Conference	33rd Biennial Conference of the Australian Association of Animal Sciences
Abbreviated title	The New Face of Animal Science in Australia
Country/Territory	Australia
City	Perth
Period	01/02/21 → 03/02/21
Other	It is with enormous pleasure that we invite you to Perth, Western Australia for the 33rd conference of the Australian Association of Animal Sciences (formerly Australian Society of Animal Production), which will be held at the Esplanade Hotel in the beautiful city of Fremantle, 1st – 4th February, 2021. For the last 60+ years this conference has brought together animal production scientists, consultants, extension specialists, producers and students to share information – from blue sky research to practical outcomes. The cross-species forum has allowed researchers to glean knowledge and ideas from outside of their areas of reference and provided an opportunity to communicate with a broad range of animal production specialists.
Internet address	http://www.animalscienceconference.com.au/ https://www.publish.csiro.au/AN/ANv61n3abs (Abstracts)

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Cite this

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title = "Prevalence of Mycoplasma bovis in a southern NSW feedlot during high and low risk periods for bovine respiratory disease",

abstract = "Bovine respiratory disease (BRD) is a multifactorial disorder in which the proliferation of bacterial pathogens may be influenced by pre-exposure to viral pathogens and behavioural and environmental stressors. The role of Mycoplasma bovis in the pathogenicity of BRD, as a non-commensal bacterium, is poorly understood and its recalcitrant nature complicates the detection in clinical samples. To overcome the limitations of conventional culture, we have utilised an absolute quantitative PCR approach (Kishimoto et al. 2017) to improve the detection of M. bovis in feedlot steers. To better understand the prevalence of this organism in the Australian feedlot system, we compared induction to hospital pen animals using qPCR and compared two DNA extraction techniques.",

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year = "2021",

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pages = "cxci",

note = "33rd Biennial Conference of the Australian Association of Animal Sciences, The New Face of Animal Science in Australia ; Conference date: 01-02-2021 Through 03-02-2021",

url = "http://www.animalscienceconference.com.au/, https://www.publish.csiro.au/AN/ANv61n3abs",

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Prevalence of Mycoplasma bovis in a southern NSW feedlot during high and low risk periods for bovine respiratory disease. / Barnewall, Rebecca; Marsh, Ian; Sales, Narelle et al.
2021. cxci Abstract from 33rd Biennial Conference of the Australian Association of Animal Sciences, Perth, Western Australia, Australia.

Research output: Other contribution to conference › Abstract › peer-review

TY - CONF

T1 - Prevalence of Mycoplasma bovis in a southern NSW feedlot during high and low risk periods for bovine respiratory disease

AU - Barnewall, Rebecca

AU - Marsh, Ian

AU - Sales, Narelle

AU - Stroud, L

AU - Williams, Thom

AU - Campbell, Michael

AU - Cusack, Paul

AU - Quinn, Jane

N1 - Conference code: 33

PY - 2021/1

Y1 - 2021/1

N2 - Bovine respiratory disease (BRD) is a multifactorial disorder in which the proliferation of bacterial pathogens may be influenced by pre-exposure to viral pathogens and behavioural and environmental stressors. The role of Mycoplasma bovis in the pathogenicity of BRD, as a non-commensal bacterium, is poorly understood and its recalcitrant nature complicates the detection in clinical samples. To overcome the limitations of conventional culture, we have utilised an absolute quantitative PCR approach (Kishimoto et al. 2017) to improve the detection of M. bovis in feedlot steers. To better understand the prevalence of this organism in the Australian feedlot system, we compared induction to hospital pen animals using qPCR and compared two DNA extraction techniques.

AB - Bovine respiratory disease (BRD) is a multifactorial disorder in which the proliferation of bacterial pathogens may be influenced by pre-exposure to viral pathogens and behavioural and environmental stressors. The role of Mycoplasma bovis in the pathogenicity of BRD, as a non-commensal bacterium, is poorly understood and its recalcitrant nature complicates the detection in clinical samples. To overcome the limitations of conventional culture, we have utilised an absolute quantitative PCR approach (Kishimoto et al. 2017) to improve the detection of M. bovis in feedlot steers. To better understand the prevalence of this organism in the Australian feedlot system, we compared induction to hospital pen animals using qPCR and compared two DNA extraction techniques.

KW - 101985

M3 - Abstract

SP - cxci

T2 - 33rd Biennial Conference of the Australian Association of Animal Sciences

Y2 - 1 February 2021 through 3 February 2021

ER -