Profiling polyphenol composition and antioxidant activity in Australian-grown rice using UHPLC Online-ABTS system

Polyphenols in Australian Rice Varieties

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Abstract

The aim of this study was to profile the polyphenol composition and antioxidant activity of 8 Australian-grown rice varieties. UHPLC with an online ABTS system was used to identify and quantify individual polyphenols present in the rice extract whilst simultaneously measuring their respective antioxidant activity. In addition, the synergistic antioxidant activity of the rice extracts was assessed using the ferric reducing ability of plasma assay (FRAP). The study found that the pigmented rice varieties had higher total antioxidant activity than the brown non-pigmented rice varieties. The red variety Yunlu29 had the highest FRAP total antioxidant activity (303.8 ± 16.16 mg 100 g-1 Trolox equivalents). UHPLC profile of Yunlu29 revealed a wide variety of active polyphenols. In particular, the chromatogram from the red rice variety Yunlu29, contained two unidentified peaks that exhibited the highest antioxidant activity. In the purple rice extract, cyanidin-3-glucoside and peonidin-3-glucoside were profiled as having the most free-radical scavenging activity. In comparison, the brown non-pigmented rice extracts did not exhibit substantial antioxidant activity in its polyphenol UHPLC profile. The results of this study demonstrate the antioxidant potential of polyphenols present in Australian-grown pigmented and non-pigmented rice varieties which may have a future role as a potential functional food ingredient.
Original languageEnglish
Pages (from-to)174-179
Number of pages6
JournalJournal of Cereal Science
Volume80
DOIs
Publication statusPublished - 03 Mar 2018

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Online Systems
Online systems
Polyphenols
polyphenols
Antioxidants
antioxidant activity
rice
Chemical analysis
extracts
glucosides
Assays
red rice
Plasmas
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
Oryza
Scavenging
cyanidin
Functional Food
assays
functional foods

Grant Number

  • IC140100027

Cite this

@article{7ad6888d4b354e7fb5c2bd221617b65a,
title = "Profiling polyphenol composition and antioxidant activity in Australian-grown rice using UHPLC Online-ABTS system: Polyphenols in Australian Rice Varieties",
abstract = "The aim of this study was to profile the polyphenol composition and antioxidant activity of 8 Australian-grown rice varieties. UHPLC with an online ABTS system was used to identify and quantify individual polyphenols present in the rice extract whilst simultaneously measuring their respective antioxidant activity. In addition, the synergistic antioxidant activity of the rice extracts was assessed using the ferric reducing ability of plasma assay (FRAP). The study found that the pigmented rice varieties had higher total antioxidant activity than the brown non-pigmented rice varieties. The red variety Yunlu29 had the highest FRAP total antioxidant activity (303.8 ± 16.16 mg 100 g-1 Trolox equivalents). UHPLC profile of Yunlu29 revealed a wide variety of active polyphenols. In particular, the chromatogram from the red rice variety Yunlu29, contained two unidentified peaks that exhibited the highest antioxidant activity. In the purple rice extract, cyanidin-3-glucoside and peonidin-3-glucoside were profiled as having the most free-radical scavenging activity. In comparison, the brown non-pigmented rice extracts did not exhibit substantial antioxidant activity in its polyphenol UHPLC profile. The results of this study demonstrate the antioxidant potential of polyphenols present in Australian-grown pigmented and non-pigmented rice varieties which may have a future role as a potential functional food ingredient.",
keywords = "Anthocyanin, Phenolic Acid, UHPLC-online ABTS, Pigmented rice,, Proanthocyanidins",
author = "Shiwangni Rao and Esther Callcott and Abishek Santhakumar and Kenneth Chinkwo and Thiru Vanniasinkam and Jixun Luo and Christopher Blanchard",
year = "2018",
month = "3",
day = "3",
doi = "10.1016/j.jcs.2018.02.011",
language = "English",
volume = "80",
pages = "174--179",
journal = "Journal of Cereal Science",
issn = "0733-5210",
publisher = "Academic Press",

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TY - JOUR

T1 - Profiling polyphenol composition and antioxidant activity in Australian-grown rice using UHPLC Online-ABTS system

T2 - Polyphenols in Australian Rice Varieties

AU - Rao, Shiwangni

AU - Callcott, Esther

AU - Santhakumar, Abishek

AU - Chinkwo, Kenneth

AU - Vanniasinkam, Thiru

AU - Luo, Jixun

AU - Blanchard, Christopher

PY - 2018/3/3

Y1 - 2018/3/3

N2 - The aim of this study was to profile the polyphenol composition and antioxidant activity of 8 Australian-grown rice varieties. UHPLC with an online ABTS system was used to identify and quantify individual polyphenols present in the rice extract whilst simultaneously measuring their respective antioxidant activity. In addition, the synergistic antioxidant activity of the rice extracts was assessed using the ferric reducing ability of plasma assay (FRAP). The study found that the pigmented rice varieties had higher total antioxidant activity than the brown non-pigmented rice varieties. The red variety Yunlu29 had the highest FRAP total antioxidant activity (303.8 ± 16.16 mg 100 g-1 Trolox equivalents). UHPLC profile of Yunlu29 revealed a wide variety of active polyphenols. In particular, the chromatogram from the red rice variety Yunlu29, contained two unidentified peaks that exhibited the highest antioxidant activity. In the purple rice extract, cyanidin-3-glucoside and peonidin-3-glucoside were profiled as having the most free-radical scavenging activity. In comparison, the brown non-pigmented rice extracts did not exhibit substantial antioxidant activity in its polyphenol UHPLC profile. The results of this study demonstrate the antioxidant potential of polyphenols present in Australian-grown pigmented and non-pigmented rice varieties which may have a future role as a potential functional food ingredient.

AB - The aim of this study was to profile the polyphenol composition and antioxidant activity of 8 Australian-grown rice varieties. UHPLC with an online ABTS system was used to identify and quantify individual polyphenols present in the rice extract whilst simultaneously measuring their respective antioxidant activity. In addition, the synergistic antioxidant activity of the rice extracts was assessed using the ferric reducing ability of plasma assay (FRAP). The study found that the pigmented rice varieties had higher total antioxidant activity than the brown non-pigmented rice varieties. The red variety Yunlu29 had the highest FRAP total antioxidant activity (303.8 ± 16.16 mg 100 g-1 Trolox equivalents). UHPLC profile of Yunlu29 revealed a wide variety of active polyphenols. In particular, the chromatogram from the red rice variety Yunlu29, contained two unidentified peaks that exhibited the highest antioxidant activity. In the purple rice extract, cyanidin-3-glucoside and peonidin-3-glucoside were profiled as having the most free-radical scavenging activity. In comparison, the brown non-pigmented rice extracts did not exhibit substantial antioxidant activity in its polyphenol UHPLC profile. The results of this study demonstrate the antioxidant potential of polyphenols present in Australian-grown pigmented and non-pigmented rice varieties which may have a future role as a potential functional food ingredient.

KW - Anthocyanin, Phenolic Acid, UHPLC-online ABTS, Pigmented rice,

KW - Proanthocyanidins

U2 - 10.1016/j.jcs.2018.02.011

DO - 10.1016/j.jcs.2018.02.011

M3 - Article

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EP - 179

JO - Journal of Cereal Science

JF - Journal of Cereal Science

SN - 0733-5210

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