Profiling volatile organic compounds produced by Bacillus species with biocontrol properties against Leptosphaeria maculans

Research output: Other contribution to conferenceAbstract

Abstract

Antifungal volatile organic compounds (VOCs) produced by Bacillus species have demonstrated their efficacy in a number of pathogen systems. Strains of Bacillus spp. were selected based on their antagonistic potential toward previously characterised virulence strains of Leptosphaeria maculans in vitro. VOCs produced by the Bacillus strains were assessed for their efficacy against L. maculans. HS-SPME-GCMS was used to profile the two best performing strains, both of which were later identified as Bacillus amyloliquefaciens based on multigene analysis. A less effective strain of B. subtilis was also used for comparison and detection of key VOCs responsible for antagonistic ability of the potential biocontrol agents. Gas chromatographic (GC-MS) studies revealed the presence of antagonistic VOCs from a range of classes, including alcohols, aldehydes, hydrocarbons and variety of ketones. Detected compounds included 2-Pentanone, 3-methyl, 2-Hexanone, 5-methyl-, Pyrazine, 2,5-dimethyl, 2-heptanone 5-methyl, 2-heptanone Benzaldehyde, 2,5-bis[(trimethylsilyl)oxy]-,2-Hexadecanol, 2,5-Cyclohexadiene-1,4-dione, 2,6-bis(1,1-dimethylethyl)-,2-Decanone,2-Decanol Cyclohexane, [(1-methylpropyl)thio]-, 6-methyl, 2-heptanone,3-Octanone,2-ethyl, 1-Hexanol, 2-Nonanone. Based on the correlation matrix, multivariate analysis was carried out using principle component analysis to identify strain differences in the production of VOCs and to determine the relatedness among the potential biocontrol strains of B. amyloliquefaciens. Additional compounds such as ethanol and hexadecane were only detected in the head space of cultures containing both the selected Bacillus strains and L. maculans, which established that the profile of VOCs differs in the presence of the fungal pathogen. In planta assays of bacterial volatiles produced by selected strains reduced disease severity by 75-85%. It is expected that profiling of VOCs from selected bacterial species may provide the opportunity to develop new tools for integrated management of blackleg disease of canola.

Original languageEnglish
Pages265
Number of pages1
Publication statusPublished - 25 Nov 2019
EventAustralasian Plant Pathology Society Conference - Melbourne Convention and Exhibition Centre, Melbourne, Australia
Duration: 26 Nov 201928 Nov 2019
https://www.apps2019.org/
https://researchoutput.csu.edu.au/admin/files/43820252/APP_2019_Program_Book_WEB_003_.pdf (Conference program)

Conference

ConferenceAustralasian Plant Pathology Society Conference
Abbreviated titleStrong Foundations, Future Innovations
CountryAustralia
CityMelbourne
Period26/11/1928/11/19
Internet address

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