TY - JOUR
T1 - Programming schistosomes - a crisper approach to transgenesis
AU - Kalinna, BH
N1 - Publisher Copyright:
© 2023 Elsevier Ltd
PY - 2023/11
Y1 - 2023/11
N2 - Ittiprasert and colleagues identified genomic safe harbour (GSH) sites in Schistosoma mansoni using computational methods and inserted a transgene into one of the sites through clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-assisted homology-directed repair. This study outlines a promising strategy for functional genomics to study this parasite that causes a debilitating and neglected tropical disease.
AB - Ittiprasert and colleagues identified genomic safe harbour (GSH) sites in Schistosoma mansoni using computational methods and inserted a transgene into one of the sites through clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-assisted homology-directed repair. This study outlines a promising strategy for functional genomics to study this parasite that causes a debilitating and neglected tropical disease.
KW - genome safe harbour site
KW - Schistosoma mansoni
KW - CRISPR/Cas9
KW - parasitic helminth transgenesis
KW - homology-directed repair
UR - http://www.scopus.com/inward/record.url?scp=85171294470&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85171294470&partnerID=8YFLogxK
U2 - 10.1016/j.pt.2023.09.003
DO - 10.1016/j.pt.2023.09.003
M3 - Comment/debate
C2 - 37718190
SN - 0169-4758
VL - 39
SP - 896
EP - 897
JO - Trends in Parasitology
JF - Trends in Parasitology
IS - 11
ER -