Protein structure determination using a combination of cross-linking, mass spectrometry, and molecular modeling

Dmitri Mouradov; Gordon King; Ian L. Ross; Jade Forwood; David Hume; Andrea Sinz; Jennifer L. Martin; Kobe Bostjan; Thomas Huber

doi:10.1007/978-1-60327-058-8_31

Protein structure determination using a combination of cross-linking, mass spectrometry, and molecular modeling

Dmitri Mouradov, Gordon King, Ian L. Ross, Jade Forwood, David Hume, Andrea Sinz, Jennifer L. Martin, Kobe Bostjan, Thomas Huber

Research output: Book chapter/Published conference paper › Chapter

17 Citations (Scopus)

Abstract

Cross-linking in combination with mass spectrometry can be used as a tool for structural modeling of protein complexes and multidomain proteins. Although cross-links represent only weak structural constraints, the combination of a limited set of experimental cross-links with molecular docking/modeling is often sufficient to determine the structure of a protein complex or multidomain protein at low resolution.

Original language	English
Title of host publication	Structural proteomics
Subtitle of host publication	high-throughput methods
Place of Publication	Totowa, N.J.
Publisher	Humana Press
Pages	459-474
Number of pages	16
Volume	426
Edition	31
ISBN (Print)	9781588298096
DOIs	https://doi.org/10.1007/978-1-60327-058-8_31
Publication status	Published - 2008

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10.1007/978-1-60327-058-8_31

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Mouradov, D., King, G., Ross, I. L., Forwood, J., Hume, D., Sinz, A., Martin, J. L., Bostjan, K., & Huber, T. (2008). Protein structure determination using a combination of cross-linking, mass spectrometry, and molecular modeling. In Structural proteomics: high-throughput methods (31 ed., Vol. 426, pp. 459-474). Humana Press. https://doi.org/10.1007/978-1-60327-058-8_31

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abstract = "Cross-linking in combination with mass spectrometry can be used as a tool for structural modeling of protein complexes and multidomain proteins. Although cross-links represent only weak structural constraints, the combination of a limited set of experimental cross-links with molecular docking/modeling is often sufficient to determine the structure of a protein complex or multidomain protein at low resolution.",

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AU - Mouradov, Dmitri

AU - King, Gordon

AU - Ross, Ian L.

AU - Forwood, Jade

AU - Hume, David

AU - Sinz, Andrea

AU - Martin, Jennifer L.

AU - Bostjan, Kobe

AU - Huber, Thomas

N1 - Imported on 12 May 2017 - DigiTool details were: publisher = Totowa, N.J: Humana Press, 2008. editor/s (773b) = Bostjan Hobe, Mitchell Guss, Thomas Huber ; Volume no. (773r) = 426; Issue no. (773s) = 31; Parent title (773t) = Structural proteomics : high-throughput methods; No. of chapters (773w) = 39.

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AB - Cross-linking in combination with mass spectrometry can be used as a tool for structural modeling of protein complexes and multidomain proteins. Although cross-links represent only weak structural constraints, the combination of a limited set of experimental cross-links with molecular docking/modeling is often sufficient to determine the structure of a protein complex or multidomain protein at low resolution.

KW - Proteomics

U2 - 10.1007/978-1-60327-058-8_31

DO - 10.1007/978-1-60327-058-8_31

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BT - Structural proteomics

PB - Humana Press

CY - Totowa, N.J.

ER -

Protein structure determination using a combination of cross-linking, mass spectrometry, and molecular modeling

Abstract

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