Pseudomonas syringae pv. maculicola in Australia: pathogenic, phenotypic and genetic diversity

Belinda Peters; Gavin Ash; Eric J Cother; Deb L Hailsones; Dorothy H Noble; Nigel Urwin

doi:10.1111/j.1365-3059.2004.00946.x

Pseudomonas syringae pv. maculicola in Australia: pathogenic, phenotypic and genetic diversity

Belinda Peters, Gavin Ash, Eric J Cother, Deb L Hailsones, Dorothy H Noble, Nigel Urwin

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Pseudomonas syringae pv. maculicola causes bacterial leaf spot on cruciferous plants in Australia. This is the first record confirming the identity of seven isolates currently stored as P. syringae pv. maculicola in the herbarium of the Department of Agriculture, Orange, Australia (Herb. DAR). The isolates were identified using pathogenicity testing on cauliflower and fatty acid methyl ester analysis. They clustered together using repetitive sequence-based polymerase chain reaction (rep-PCR) and PCR-restriction fragment-length polymorphism (RFLP) of the 16S'23S internal transcribed spacer region (ITS) using seven restriction enzymes. Identification was confirmed by comparison of these isolates with known overseas isolates of P. syringae pv. maculicola, but there was significant variation in their pathogenicity and genetic structure.

Original language	English
Pages (from-to)	73-79
Number of pages	7
Journal	Plant Pathology
Volume	53
Issue number	1
DOIs	https://doi.org/10.1111/j.1365-3059.2004.00946.x
Publication status	Published - 2004

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title = "Pseudomonas syringae pv. maculicola in Australia: pathogenic, phenotypic and genetic diversity",

abstract = "Pseudomonas syringae pv. maculicola causes bacterial leaf spot on cruciferous plants in Australia. This is the first record confirming the identity of seven isolates currently stored as P. syringae pv. maculicola in the herbarium of the Department of Agriculture, Orange, Australia (Herb. DAR). The isolates were identified using pathogenicity testing on cauliflower and fatty acid methyl ester analysis. They clustered together using repetitive sequence-based polymerase chain reaction (rep-PCR) and PCR-restriction fragment-length polymorphism (RFLP) of the 16S'23S internal transcribed spacer region (ITS) using seven restriction enzymes. Identification was confirmed by comparison of these isolates with known overseas isolates of P. syringae pv. maculicola, but there was significant variation in their pathogenicity and genetic structure.",

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AU - Peters, Belinda

AU - Ash, Gavin

AU - Cother, Eric J

AU - Hailsones, Deb L

AU - Noble, Dorothy H

AU - Urwin, Nigel

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AB - Pseudomonas syringae pv. maculicola causes bacterial leaf spot on cruciferous plants in Australia. This is the first record confirming the identity of seven isolates currently stored as P. syringae pv. maculicola in the herbarium of the Department of Agriculture, Orange, Australia (Herb. DAR). The isolates were identified using pathogenicity testing on cauliflower and fatty acid methyl ester analysis. They clustered together using repetitive sequence-based polymerase chain reaction (rep-PCR) and PCR-restriction fragment-length polymorphism (RFLP) of the 16S'23S internal transcribed spacer region (ITS) using seven restriction enzymes. Identification was confirmed by comparison of these isolates with known overseas isolates of P. syringae pv. maculicola, but there was significant variation in their pathogenicity and genetic structure.

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Pseudomonas syringae pv. maculicola in Australia: pathogenic, phenotypic and genetic diversity

Abstract

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