Pulsed-amperometric detection of urea in blood samples on a conducting polypyrrole-urease biosensor

S. B. Adeloju; S. J. Shaw; G. G. Wallace

doi:10.1016/S0003-2670(96)00502-8

Pulsed-amperometric detection of urea in blood samples on a conducting polypyrrole-urease biosensor

S. B. Adeloju, S. J. Shaw, G. G. Wallace

Science and Health

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

Abstract

A rapid and highly sensitive method is described for reliable flow injection analysis of urea based on pulsed-amperometric detection with a polypyrrole-urease biosensor. The optimum conditions were 0.05 M phosphate buffer (pH 7.0), a negative potential pulse between -70 mV (E ₁) and -400 mV (E ₂) VS Ag/AgCl, a pulse width of 120 mS, and a flow rate of 0.1 mi min ^-1. A detection limit (3σ) of 60 μg l ^-1 of urea was achieved by this method and the response was linear in the range 100-4500 μg l ^-1. The biosensor was very stable in the flow system, even after two weeks of continuous use. Excellent recovery of urea (95%-112%) was accomplished employing this method in simulated samples. Successful application of the method to the determination of urea in blood plasma is reported.

Original language	English
Pages (from-to)	155-160
Number of pages	6
Journal	Analytica Chimica Acta
Volume	341
Issue number	2-3
DOIs	https://doi.org/10.1016/S0003-2670(96)00502-8
Publication status	Published - 10 Apr 1997

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title = "Pulsed-amperometric detection of urea in blood samples on a conducting polypyrrole-urease biosensor",

abstract = "A rapid and highly sensitive method is described for reliable flow injection analysis of urea based on pulsed-amperometric detection with a polypyrrole-urease biosensor. The optimum conditions were 0.05 M phosphate buffer (pH 7.0), a negative potential pulse between -70 mV (E 1) and -400 mV (E 2) VS Ag/AgCl, a pulse width of 120 mS, and a flow rate of 0.1 mi min -1. A detection limit (3σ) of 60 μg l -1 of urea was achieved by this method and the response was linear in the range 100-4500 μg l -1. The biosensor was very stable in the flow system, even after two weeks of continuous use. Excellent recovery of urea (95%-112%) was accomplished employing this method in simulated samples. Successful application of the method to the determination of urea in blood plasma is reported.",

keywords = "Amperometry, Biosensors, Blood plasma, Flow injection, Polypyrrole, Urea, Urease",

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AU - Adeloju, S. B.

AU - Shaw, S. J.

AU - Wallace, G. G.

PY - 1997/4/10

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N2 - A rapid and highly sensitive method is described for reliable flow injection analysis of urea based on pulsed-amperometric detection with a polypyrrole-urease biosensor. The optimum conditions were 0.05 M phosphate buffer (pH 7.0), a negative potential pulse between -70 mV (E 1) and -400 mV (E 2) VS Ag/AgCl, a pulse width of 120 mS, and a flow rate of 0.1 mi min -1. A detection limit (3σ) of 60 μg l -1 of urea was achieved by this method and the response was linear in the range 100-4500 μg l -1. The biosensor was very stable in the flow system, even after two weeks of continuous use. Excellent recovery of urea (95%-112%) was accomplished employing this method in simulated samples. Successful application of the method to the determination of urea in blood plasma is reported.

AB - A rapid and highly sensitive method is described for reliable flow injection analysis of urea based on pulsed-amperometric detection with a polypyrrole-urease biosensor. The optimum conditions were 0.05 M phosphate buffer (pH 7.0), a negative potential pulse between -70 mV (E 1) and -400 mV (E 2) VS Ag/AgCl, a pulse width of 120 mS, and a flow rate of 0.1 mi min -1. A detection limit (3σ) of 60 μg l -1 of urea was achieved by this method and the response was linear in the range 100-4500 μg l -1. The biosensor was very stable in the flow system, even after two weeks of continuous use. Excellent recovery of urea (95%-112%) was accomplished employing this method in simulated samples. Successful application of the method to the determination of urea in blood plasma is reported.

KW - Amperometry

KW - Biosensors

KW - Blood plasma

KW - Flow injection

KW - Polypyrrole

KW - Urea

KW - Urease

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Pulsed-amperometric detection of urea in blood samples on a conducting polypyrrole-urease biosensor

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