Purification of adenovirus and adeno-associated virus: Comparison of novel membrane-based technology to conventional techniques

A.M. Duffy, A.M. O'Doherty, T. O'Brien, P.M. Strappe

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Adenovirus (Ad) and Adeno-associated virus (AAV) are efficient gene delivery systems; manipulation of the wild-type genome allows their use as vectors for the over expression of desirable transgenes. Generation and purification of such viral vectors can be labour intensive, costly and require specialized equipment, but a new generation of membrane-mediated ion exchange kits for purification of recombinant virus may facilitate this process. Here, we examine the yields, transgene expression and purity of preparations of Ad and AAV purified using commercially available kits in comparison to other established techniques for purification of recombinant viral vectors. We demonstrate comparable results for Ad and AAV respectively in all parameters investigated, with a substantial reduction in purification time for the kit-based technology. Such approaches are attractive methods for small-scale purification of recombinant Ad and AAV viral vectors.
Original languageEnglish
Pages (from-to)62-72
Number of pages11
JournalGene Therapy
Volume12
Issue number(SUPP. 1)
DOIs
Publication statusPublished - 2005

Fingerprint Dive into the research topics of 'Purification of adenovirus and adeno-associated virus: Comparison of novel membrane-based technology to conventional techniques'. Together they form a unique fingerprint.

  • Cite this