Purple Sweet Potato Pigments Scavenge ROS, Reduce p53 and Modulate Bcl-2/Bax to Inhibit Irradiation-induced Apoptosis in Murine Thymocytes

Yan-Tao Han, Xue-Hong Chen, Jing Xie, Song-Mei Zhan, Chun-Bo Wang, Lexin Wang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Aims: Purple sweet potato (PSP) pigments were proved to protect murine thymocytes from 60Co '-ray-induced mitochondria-mediated apoptosis in our previous study. In this study, we further investigated the effect of PSP pigments on apoptosis related ROS, p53 and Bcl-2 family. Methods: Cell viability was analyzed by MTT. Apoptosis was certified by DNA ladder detection. Reactive oxygen species (ROS) were detected using 2',7',- dichlorofluorescein diacetate (DCFH-DA) probe. P53, Bcl-2 and Bax proteins were analyzed by western blot. The activities of caspase-3 and caspase-9 were determined by fluorogenic substrates detection. Results: PSP pigments treatment prior to 4Gy 60Co '-ray irradiation increased the cell viability and decrease the apoptosis. In the presence of PSP pigments, ROS was scavenged and followed by a p53-depression. A shift in Bcl-2/Bax ratio towards anti-apoptosis was observed as a result of p53-depression. The activities of caspase-9 and caspase-3 were reduced by PSP pigments pretreatment. Conclusions: PSP pigments have a cytoprotective activity against ' radiation. The protective effect of PSP pigments may be involving ROS scavenging, p53 depression and Bcl-2/Bax modulation in a caspase-dependent mitochondrial way.
Original languageEnglish
Pages (from-to)865-872
Number of pages8
JournalCellular Physiology and Biochemistry
Volume28
Issue number5
DOIs
Publication statusPublished - 2011

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Ipomoea batatas
Thymocytes
Reactive Oxygen Species
Apoptosis
Caspase 9
Caspase 3
Cell Survival
bcl-2-Associated X Protein
Caspases
Fluorescent Dyes
Mitochondria
Western Blotting
Radiation
DNA

Cite this

@article{d09bf7ce26664bc9b1db71111465c6d1,
title = "Purple Sweet Potato Pigments Scavenge ROS, Reduce p53 and Modulate Bcl-2/Bax to Inhibit Irradiation-induced Apoptosis in Murine Thymocytes",
abstract = "Aims: Purple sweet potato (PSP) pigments were proved to protect murine thymocytes from 60Co '-ray-induced mitochondria-mediated apoptosis in our previous study. In this study, we further investigated the effect of PSP pigments on apoptosis related ROS, p53 and Bcl-2 family. Methods: Cell viability was analyzed by MTT. Apoptosis was certified by DNA ladder detection. Reactive oxygen species (ROS) were detected using 2',7',- dichlorofluorescein diacetate (DCFH-DA) probe. P53, Bcl-2 and Bax proteins were analyzed by western blot. The activities of caspase-3 and caspase-9 were determined by fluorogenic substrates detection. Results: PSP pigments treatment prior to 4Gy 60Co '-ray irradiation increased the cell viability and decrease the apoptosis. In the presence of PSP pigments, ROS was scavenged and followed by a p53-depression. A shift in Bcl-2/Bax ratio towards anti-apoptosis was observed as a result of p53-depression. The activities of caspase-9 and caspase-3 were reduced by PSP pigments pretreatment. Conclusions: PSP pigments have a cytoprotective activity against ' radiation. The protective effect of PSP pigments may be involving ROS scavenging, p53 depression and Bcl-2/Bax modulation in a caspase-dependent mitochondrial way.",
keywords = "Apoptosis, Bcl-2/Bax, Purple sweet potato (PSP) pigments, ROS, ' irradiation",
author = "Yan-Tao Han and Xue-Hong Chen and Jing Xie and Song-Mei Zhan and Chun-Bo Wang and Lexin Wang",
note = "Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = Cellular Physiology and Biochemistry. ISSNs: 1015-8987;",
year = "2011",
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Purple Sweet Potato Pigments Scavenge ROS, Reduce p53 and Modulate Bcl-2/Bax to Inhibit Irradiation-induced Apoptosis in Murine Thymocytes. / Han, Yan-Tao; Chen, Xue-Hong; Xie, Jing; Zhan, Song-Mei; Wang, Chun-Bo; Wang, Lexin.

In: Cellular Physiology and Biochemistry, Vol. 28, No. 5, 2011, p. 865-872.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Purple Sweet Potato Pigments Scavenge ROS, Reduce p53 and Modulate Bcl-2/Bax to Inhibit Irradiation-induced Apoptosis in Murine Thymocytes

AU - Han, Yan-Tao

AU - Chen, Xue-Hong

AU - Xie, Jing

AU - Zhan, Song-Mei

AU - Wang, Chun-Bo

AU - Wang, Lexin

N1 - Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = Cellular Physiology and Biochemistry. ISSNs: 1015-8987;

PY - 2011

Y1 - 2011

N2 - Aims: Purple sweet potato (PSP) pigments were proved to protect murine thymocytes from 60Co '-ray-induced mitochondria-mediated apoptosis in our previous study. In this study, we further investigated the effect of PSP pigments on apoptosis related ROS, p53 and Bcl-2 family. Methods: Cell viability was analyzed by MTT. Apoptosis was certified by DNA ladder detection. Reactive oxygen species (ROS) were detected using 2',7',- dichlorofluorescein diacetate (DCFH-DA) probe. P53, Bcl-2 and Bax proteins were analyzed by western blot. The activities of caspase-3 and caspase-9 were determined by fluorogenic substrates detection. Results: PSP pigments treatment prior to 4Gy 60Co '-ray irradiation increased the cell viability and decrease the apoptosis. In the presence of PSP pigments, ROS was scavenged and followed by a p53-depression. A shift in Bcl-2/Bax ratio towards anti-apoptosis was observed as a result of p53-depression. The activities of caspase-9 and caspase-3 were reduced by PSP pigments pretreatment. Conclusions: PSP pigments have a cytoprotective activity against ' radiation. The protective effect of PSP pigments may be involving ROS scavenging, p53 depression and Bcl-2/Bax modulation in a caspase-dependent mitochondrial way.

AB - Aims: Purple sweet potato (PSP) pigments were proved to protect murine thymocytes from 60Co '-ray-induced mitochondria-mediated apoptosis in our previous study. In this study, we further investigated the effect of PSP pigments on apoptosis related ROS, p53 and Bcl-2 family. Methods: Cell viability was analyzed by MTT. Apoptosis was certified by DNA ladder detection. Reactive oxygen species (ROS) were detected using 2',7',- dichlorofluorescein diacetate (DCFH-DA) probe. P53, Bcl-2 and Bax proteins were analyzed by western blot. The activities of caspase-3 and caspase-9 were determined by fluorogenic substrates detection. Results: PSP pigments treatment prior to 4Gy 60Co '-ray irradiation increased the cell viability and decrease the apoptosis. In the presence of PSP pigments, ROS was scavenged and followed by a p53-depression. A shift in Bcl-2/Bax ratio towards anti-apoptosis was observed as a result of p53-depression. The activities of caspase-9 and caspase-3 were reduced by PSP pigments pretreatment. Conclusions: PSP pigments have a cytoprotective activity against ' radiation. The protective effect of PSP pigments may be involving ROS scavenging, p53 depression and Bcl-2/Bax modulation in a caspase-dependent mitochondrial way.

KW - Apoptosis

KW - Bcl-2/Bax

KW - Purple sweet potato (PSP) pigments

KW - ROS

KW - ' irradiation

U2 - 10.1159/000335801

DO - 10.1159/000335801

M3 - Article

VL - 28

SP - 865

EP - 872

JO - Cellular Physiology and Biochemistry

JF - Cellular Physiology and Biochemistry

SN - 1015-8987

IS - 5

ER -