TY - JOUR
T1 - Q-TOF LC/MS identification and UHPLC-Online ABTS antioxidant activity guided mapping of barley polyphenols
AU - Rao, Shiwangni
AU - Santhakumar, Abishek B.
AU - Chinkwo, Kenneth A.
AU - Blanchard, Christopher L.
N1 - Includes bibliographical references.
PY - 2018/11/15
Y1 - 2018/11/15
N2 - The polyphenol composition and antioxidant activity of seven Australian-grown barley varieties were characterized in this study. UHPLC with an online ABTS system was used to identify individual polyphenols while simultaneously measuring their antioxidant activity. The Q-TOF LC/MS system was utilized to identify the phenolic compounds that demonstrated substantial antioxidant activity. The variety, Hindmarsh, showed the highest total phenolic content and antioxidant activity. There was no significant difference observed amongst the other varieties in their total phenolic content, however, they did have significant variation in proanthocyanidin content and antioxidant activity (p < 0.05). Prodelphinidin B3 was the most abundant polyphenol with the highest antioxidant activity amongst all the barley varieties tested. Other polyphenols identified with antioxidant activity included procyanidin, glycosides of catechin and flavan-3-ols. Polyphenol characterization of Australian grown barley varieties demonstrated that they have significant antioxidant activity, hence, promoting the value of whole grain barley as a potential functional food ingredient.
AB - The polyphenol composition and antioxidant activity of seven Australian-grown barley varieties were characterized in this study. UHPLC with an online ABTS system was used to identify individual polyphenols while simultaneously measuring their antioxidant activity. The Q-TOF LC/MS system was utilized to identify the phenolic compounds that demonstrated substantial antioxidant activity. The variety, Hindmarsh, showed the highest total phenolic content and antioxidant activity. There was no significant difference observed amongst the other varieties in their total phenolic content, however, they did have significant variation in proanthocyanidin content and antioxidant activity (p < 0.05). Prodelphinidin B3 was the most abundant polyphenol with the highest antioxidant activity amongst all the barley varieties tested. Other polyphenols identified with antioxidant activity included procyanidin, glycosides of catechin and flavan-3-ols. Polyphenol characterization of Australian grown barley varieties demonstrated that they have significant antioxidant activity, hence, promoting the value of whole grain barley as a potential functional food ingredient.
KW - Antioxidant
KW - Barley
KW - Cereals
KW - Q-TOF LC/MS
KW - UHPLC-online ABTS
UR - http://www.scopus.com/inward/record.url?scp=85048245974&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85048245974&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2018.06.011
DO - 10.1016/j.foodchem.2018.06.011
M3 - Article
C2 - 30381193
AN - SCOPUS:85048245974
SN - 0308-8146
VL - 266
SP - 323
EP - 328
JO - Food Chemistry
JF - Food Chemistry
ER -