Quantitative gene profiling of long noncoding RNAs with targeted RNA sequencing

Michael B Clark, Tim R Mercer, Giovanni Bussotti, Tommaso Leonardi, Katelin R Haynes, Joanna Crawford, Marion E Brunck, Kim-Anh Lê Cao, Gethin P Thomas, Wendy Y Chen, Ryan J Taft, Lars K Nielsen, Anton J Enright, John S Mattick, Marcel E Dinger

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    94 Citations (Scopus)

    Abstract

    We compared quantitative RT-PCR (qRT-PCR), RNA-seq and capture sequencing (CaptureSeq) in terms of their ability to assemble and quantify long noncoding RNAs and novel coding exons across 20 human tissues. CaptureSeq was superior for the detection and quantification of genes with low expression, showed little technical variation and accurately measured differential expression. This approach expands and refines previous annotations and simultaneously generates an expression atlas
    Original languageEnglish
    Pages (from-to)339-342
    Number of pages4
    JournalNature Methods
    Volume12
    Issue number4
    DOIs
    Publication statusPublished - 2015

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  • Cite this

    Clark, M. B., Mercer, T. R., Bussotti, G., Leonardi, T., Haynes, K. R., Crawford, J., Brunck, M. E., Lê Cao, K-A., Thomas, G. P., Chen, W. Y., Taft, R. J., Nielsen, L. K., Enright, A. J., Mattick, J. S., & Dinger, M. E. (2015). Quantitative gene profiling of long noncoding RNAs with targeted RNA sequencing. Nature Methods, 12(4), 339-342. https://doi.org/10.1038/nmeth.3321