Rapid detection of Bovicola ovis using colourimetric loop-mediated isothermal amplification (LAMP): a potential tool for the detection of sheep lice infestation on farm

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Abstract

The sheep body louse, Bovicola ovis (B. ovis), is one of the most significant ectoparasites affecting Australia’s sheep flocks. Despite this, detection methods for B. ovis infestation are limited to visual inspection and ELISA. A colourimetric loop-mediated isothermal amplification (LAMP) method was developed and evaluated for the detection of B. ovis DNA. Diagnostic sensitivity and specificity of LAMP were compared with those of visual inspection and PCR and validated using field samples collected from 22 farms. Two different DNA extraction methods using a commercial kit and a boiling method were also compared. The
highest sensitivity and specificity were observed when PCR was used and DNAwas extracted using a commercial kit. Compared with PCR, the LAMP assay demonstrated a sensitivity and specificity of 90% and 92% when DNA was extracted by a commercial kit and 100% and 75% when DNA was extracted by the boiling method, respectively. The LAMP test developed in this study could potentially serve as a point-of-care diagnostic tool for monitoring of sheep flocks as well as surveillance of B. ovis populations.
Original languageEnglish
JournalParasitology Research
DOIs
Publication statusPublished - 11 Dec 2019

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Ischnocera
Bovicola ovis
Lice Infestations
lice infestations
Sheep
sheep
farms
DNA
boiling
flocks
Sensitivity and Specificity
diagnostic specificity
Polymerase Chain Reaction
diagnostic sensitivity
methodology
monitoring
Point-of-Care Systems
ectoparasites
Pediculus
loop-mediated isothermal amplification

Grant Number

  • 50714
  • 40702

Cite this

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title = "Rapid detection of Bovicola ovis using colourimetric loop-mediated isothermal amplification (LAMP): a potential tool for the detection of sheep lice infestation on farm",
abstract = "The sheep body louse, Bovicola ovis (B. ovis), is one of the most significant ectoparasites affecting Australia’s sheep flocks. Despite this, detection methods for B. ovis infestation are limited to visual inspection and ELISA. A colourimetric loop-mediated isothermal amplification (LAMP) method was developed and evaluated for the detection of B. ovis DNA. Diagnostic sensitivity and specificity of LAMP were compared with those of visual inspection and PCR and validated using field samples collected from 22 farms. Two different DNA extraction methods using a commercial kit and a boiling method were also compared. Thehighest sensitivity and specificity were observed when PCR was used and DNAwas extracted using a commercial kit. Compared with PCR, the LAMP assay demonstrated a sensitivity and specificity of 90{\%} and 92{\%} when DNA was extracted by a commercial kit and 100{\%} and 75{\%} when DNA was extracted by the boiling method, respectively. The LAMP test developed in this study could potentially serve as a point-of-care diagnostic tool for monitoring of sheep flocks as well as surveillance of B. ovis populations.",
author = "Simon Wong and Rob Woodgate and Sam Pant and Seyed Ghorashi",
year = "2019",
month = "12",
day = "11",
doi = "doi: 10.1007/s00436-019-06552-y",
language = "English",
journal = "Parasitology Research",
issn = "0932-0113",
publisher = "Springer-Verlag London Ltd.",

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TY - JOUR

T1 - Rapid detection of Bovicola ovis using colourimetric loop-mediated isothermal amplification (LAMP): a potential tool for the detection of sheep lice infestation on farm

AU - Wong, Simon

AU - Woodgate, Rob

AU - Pant, Sam

AU - Ghorashi, Seyed

PY - 2019/12/11

Y1 - 2019/12/11

N2 - The sheep body louse, Bovicola ovis (B. ovis), is one of the most significant ectoparasites affecting Australia’s sheep flocks. Despite this, detection methods for B. ovis infestation are limited to visual inspection and ELISA. A colourimetric loop-mediated isothermal amplification (LAMP) method was developed and evaluated for the detection of B. ovis DNA. Diagnostic sensitivity and specificity of LAMP were compared with those of visual inspection and PCR and validated using field samples collected from 22 farms. Two different DNA extraction methods using a commercial kit and a boiling method were also compared. Thehighest sensitivity and specificity were observed when PCR was used and DNAwas extracted using a commercial kit. Compared with PCR, the LAMP assay demonstrated a sensitivity and specificity of 90% and 92% when DNA was extracted by a commercial kit and 100% and 75% when DNA was extracted by the boiling method, respectively. The LAMP test developed in this study could potentially serve as a point-of-care diagnostic tool for monitoring of sheep flocks as well as surveillance of B. ovis populations.

AB - The sheep body louse, Bovicola ovis (B. ovis), is one of the most significant ectoparasites affecting Australia’s sheep flocks. Despite this, detection methods for B. ovis infestation are limited to visual inspection and ELISA. A colourimetric loop-mediated isothermal amplification (LAMP) method was developed and evaluated for the detection of B. ovis DNA. Diagnostic sensitivity and specificity of LAMP were compared with those of visual inspection and PCR and validated using field samples collected from 22 farms. Two different DNA extraction methods using a commercial kit and a boiling method were also compared. Thehighest sensitivity and specificity were observed when PCR was used and DNAwas extracted using a commercial kit. Compared with PCR, the LAMP assay demonstrated a sensitivity and specificity of 90% and 92% when DNA was extracted by a commercial kit and 100% and 75% when DNA was extracted by the boiling method, respectively. The LAMP test developed in this study could potentially serve as a point-of-care diagnostic tool for monitoring of sheep flocks as well as surveillance of B. ovis populations.

UR - https://link.springer.com/article/10.1007%2Fs00436-019-06552-y

U2 - doi: 10.1007/s00436-019-06552-y

DO - doi: 10.1007/s00436-019-06552-y

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