TY - JOUR
T1 - Rapid detection of Bovicola ovis using colourimetric loop-mediated isothermal amplification (LAMP)
T2 - A potential tool for the detection of sheep lice infestation on farm
AU - Wong, Simon
AU - Woodgate, Rob
AU - Pant, Sam
AU - Ghorashi, Seyed
PY - 2020
Y1 - 2020
N2 - The sheep body louse, Bovicola ovis (B. ovis), is one of the most significant ectoparasites affecting Australia’s sheep flocks. Despite this, detection methods for B. ovis
infestation are limited to visual inspection and ELISA. A colourimetric
loop-mediated isothermal amplification (LAMP) method was developed and
evaluated for the detection of B. ovis DNA. Diagnostic
sensitivity and specificity of LAMP were compared with those of visual
inspection and PCR and validated using field samples collected from 22
farms. Two different DNA extraction methods using a commercial kit and a
boiling method were also compared. The highest sensitivity and
specificity were observed when PCR was used and DNA was extracted using a
commercial kit. Compared with PCR, the LAMP assay demonstrated a
sensitivity and specificity of 90% and 92% when DNA was extracted by a
commercial kit and 100% and 75% when DNA was extracted by the boiling
method, respectively. The LAMP test developed in this study could
potentially serve as a point-of-care diagnostic tool for monitoring of
sheep flocks as well as surveillance of B. ovis populations.
AB - The sheep body louse, Bovicola ovis (B. ovis), is one of the most significant ectoparasites affecting Australia’s sheep flocks. Despite this, detection methods for B. ovis
infestation are limited to visual inspection and ELISA. A colourimetric
loop-mediated isothermal amplification (LAMP) method was developed and
evaluated for the detection of B. ovis DNA. Diagnostic
sensitivity and specificity of LAMP were compared with those of visual
inspection and PCR and validated using field samples collected from 22
farms. Two different DNA extraction methods using a commercial kit and a
boiling method were also compared. The highest sensitivity and
specificity were observed when PCR was used and DNA was extracted using a
commercial kit. Compared with PCR, the LAMP assay demonstrated a
sensitivity and specificity of 90% and 92% when DNA was extracted by a
commercial kit and 100% and 75% when DNA was extracted by the boiling
method, respectively. The LAMP test developed in this study could
potentially serve as a point-of-care diagnostic tool for monitoring of
sheep flocks as well as surveillance of B. ovis populations.
UR - https://link.springer.com/article/10.1007%2Fs00436-019-06552-y
U2 - 10.1007/s00436-019-06552-y
DO - 10.1007/s00436-019-06552-y
M3 - Article
C2 - 31820169
SN - 0932-0113
VL - 119
SP - 395
EP - 401
JO - Parasitology Research
JF - Parasitology Research
ER -