Rapid fabrication of homogeneously distributed hyper-branched gold nanostructured electrode based electrochemical immunosensor for detection of protein biomarkers

Shrishty Bakshi, Samriddhi Mehta, Tushar Kumeria, Muhammad J.A. Shiddiky, Amirali Popat, Sangeeta Choudhury, Sudeep Bose, Ranu Nayak

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

In recent years, need for rapid, reliable, and low-cost protein biomarker screening has tremendously boosted research into the development of electrochemical immunosensors. In this line, here we report on rapid fabrication and proof-of-concept demonstration of a hyper-branched gold nanostructure (ns@gold) based electrochemical immunosensor for detection of protein biomarker at pg/mL level. We have optimized a simple direct electrochemical process to rapidly generate uniformly distributed high surface area ns@gold within 30 min. These 3D ns@gold show ∼ 8-times enhancement in conductivity and also a rapid surface functionalization via self-assembled monolayers (SAM) of mercaptoundecanoic acid within 45 min. Protein estimation data showed around 94 % immobilization efficiency on the ns@gold surface in comparison to 28.4 % on plain gold surface. A fast SAM formation and quick antibody immobilization steered the assay timing within 4 h. As a proof-of-concept, we used an antibody-antigen interaction for the detection of epithelial growth factor receptor (EGFR) protein. Voltammetric data for the target EGFR receptor resulted a linear working range of 10 pg/mL to 100 ng/mL with a low limit-of-detection of 6.9 pg/mL. High selectivity in detecting target proteins from a pool of non-target proteins using breast cancer cell lines was also demonstrated along with good stability and reusability.
Original languageEnglish
Article number128803
Pages (from-to)1-11
Number of pages11
JournalSensors and Actuators, B: Chemical
Volume326
Early online date16 Sept 2020
DOIs
Publication statusPublished - 01 Jan 2021

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