TY - JOUR
T1 - Rapid identification of Streptococcus pneumoniae serotypes by cpsB gene-based sequetyping combined with multiplex PCR
AU - Zhou, Meng Lan
AU - Wang, Zi Ran
AU - Li, Yan Bing
AU - Kudinha, Timothy
AU - Wang, Jian
AU - Wang, Yao
AU - Xiao, Meng
AU - Xu, Ying Chun
AU - Liu, Zheng Yin
AU - Hsueh, Po Ren
N1 - Funding Information:
This work was supported by the National Key Research and Development Program of China ( 2017YFC1601502 ), Special Foundation for National Science and Technology Basic Research Program of China ( 2019FY101200 ), Beijing Key Clinical Specialty for Laboratory Medicine Excellent Project (No. ZK201000 ) and the Graduate Innovation Fund of Peking Union Medical College (Grant No. 2017-1002-1-21 ).
Publisher Copyright:
© 2021
PY - 2021/11/29
Y1 - 2021/11/29
N2 - Background/purpose: Streptococcus pneumoniae is an important human pathogen that causes invasive infections in adults and children. Accurate serotyping is important to study its epidemiological distribution and to assess vaccine efficacy.Methods: Invasive S. pneumoniae isolates (n = 300) from 27 teaching hospitals in China were studied. The Quellung reaction was used as the gold standard to identify the S. pneumoniae serotypes. Subsequently, multiplex PCR and cpsB gene-based sequetyping methods were used to identify the serotypes.Methods: Based on the Quellung reaction, 299 S. pneumoniae isolates were accurately identified to the serotype level and 40 different serotypes were detected. Only one strain was non-typeable, and five most common serotypes were identified: 23F (43, 14.3%), 19A (41, 13.7%), 19F (41, 13.7%), 3 (31, 10.3%), and 14 (27, 9.0%). Overall, the multiplex PCR method identified 73.3 and 20.7% of the isolates to the serotype and cluster levels, respectively, with 1.7% of the isolates misidentified. In contrast, the cpsB sequetyping method identified 59.0 and 30.3% of the isolates to the serotype and cluster levels, respectively, and 7% were misidentified. Conclusions: The cpsB gene sequetyping method combined with multiplex PCR, can greatly improve the accuracy and efficiency of serotyping, besides reducing the associated costs.
AB - Background/purpose: Streptococcus pneumoniae is an important human pathogen that causes invasive infections in adults and children. Accurate serotyping is important to study its epidemiological distribution and to assess vaccine efficacy.Methods: Invasive S. pneumoniae isolates (n = 300) from 27 teaching hospitals in China were studied. The Quellung reaction was used as the gold standard to identify the S. pneumoniae serotypes. Subsequently, multiplex PCR and cpsB gene-based sequetyping methods were used to identify the serotypes.Methods: Based on the Quellung reaction, 299 S. pneumoniae isolates were accurately identified to the serotype level and 40 different serotypes were detected. Only one strain was non-typeable, and five most common serotypes were identified: 23F (43, 14.3%), 19A (41, 13.7%), 19F (41, 13.7%), 3 (31, 10.3%), and 14 (27, 9.0%). Overall, the multiplex PCR method identified 73.3 and 20.7% of the isolates to the serotype and cluster levels, respectively, with 1.7% of the isolates misidentified. In contrast, the cpsB sequetyping method identified 59.0 and 30.3% of the isolates to the serotype and cluster levels, respectively, and 7% were misidentified. Conclusions: The cpsB gene sequetyping method combined with multiplex PCR, can greatly improve the accuracy and efficiency of serotyping, besides reducing the associated costs.
KW - cpsB sequetyping
KW - Multiplex PCR
KW - Quellung reaction
KW - Serotype
KW - Streptococcus pneumoniae
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U2 - 10.1016/j.jmii.2021.11.004
DO - 10.1016/j.jmii.2021.11.004
M3 - Article
C2 - 34924338
AN - SCOPUS:85121299405
SN - 0253-2662
SP - 1
EP - 10
JO - Journal of Microbiology, Immunology and Infection
JF - Journal of Microbiology, Immunology and Infection
ER -
