Rapid solid-phase extraction and analysis of resveratrol and other polyphenols in red wine

Shima NNS Hashim, Lachlan J Schwarz, Reinhard I Boysen, Yuanzhong Yang, Basil Danylec, Milton TW Hearn

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Red wine has long been credited as a good source of health-beneficial antioxidants, including the bioactive polyphenols catechin, quercetin, and (E)-resveratrol. In this paper, we report the application of reusable molecularly imprinted polymers (MIPs) for the selective and robust solid-phase extraction (SPE) and rapid analysis of (E)-resveratrol (LOD = 8.87 × 10−3 mg/L, LOQ = 2.94 × 10−2 mg/L), along with a range of other polyphenols from an Australian Pinot noir red wine. Optimization of the molecularly imprinted solid-phase extraction (MISPE) protocol resulted in the significant enrichment of (E)-resveratrol and several structurally related polyphenols. These secondary metabolites were subsequently identified by RP-HPLC and μLC–ESI ion trap MS/MS methods. The developed MISPE protocol employed low volumes of environmentally benign solvents selected according to the Green Chemistry principles, and resulted in the recovery of 99% of the total (E)-resveratrol present. These results further demonstrate the potential of generic protocols for the analysis of target compound with health beneficial properties within the food and nutraceutical industries using tailor-made MIPs.
Original languageEnglish
Pages (from-to)284-290
Number of pages7
JournalJournal of Chromatography A
Volume1313
DOIs
Publication statusPublished - 2013

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