Abstract
Coumestans are naturally occurring, non-steroidal, plant-derived compounds formed from cinnamic acid, that have been observed to act as oestrogen antagonists in cattle and other grazing livestock. Coumestans may negatively affect the reproductive functions of cattle at concentrations greater than 18 mg/kg DM in pasture [1].
It has been found that long term ingestion of coumestans in mares caused a drop in blood progesterone and a high coumestan concentration in the blood [2]. This in turn affected their resulting cyclicity, interfered with ovulation, and caused uterine oedema and uterine fluid accumulation, adversely affecting conception and embryo development [2].
Coumestrol and its metabolites methoxycoumestrol have also been identified in the plasma of domesticated animals such as sheep, horses, goats and cattle [1]. In 2019, we identified the metabolite 3’methoxycoumestrol in bovine plasma [3]. In 2023, coumestrin, a glucoside of coumestrol has also been identified in bovine plasma using a sensitive metabolite profiling method employing UPLC-QToF-MS and UPLC-QQQ-MS analysis. Over time, we have identified precurors of plant-based coumestans in lucerne and other legume crops and are now tracking key metabolites of abundance in bovine plasma over time in replicated feeding trials. We are also correlating these results with reproductive function.
1.Reed K; Agriculture 2016, 6(3), 35.
2.Ferreira-Dias, et al.; Theriogenology 2013, 80, 684-692.
3.Wyse, et al.; Metabolites 2021, 11(8), 550.
It has been found that long term ingestion of coumestans in mares caused a drop in blood progesterone and a high coumestan concentration in the blood [2]. This in turn affected their resulting cyclicity, interfered with ovulation, and caused uterine oedema and uterine fluid accumulation, adversely affecting conception and embryo development [2].
Coumestrol and its metabolites methoxycoumestrol have also been identified in the plasma of domesticated animals such as sheep, horses, goats and cattle [1]. In 2019, we identified the metabolite 3’methoxycoumestrol in bovine plasma [3]. In 2023, coumestrin, a glucoside of coumestrol has also been identified in bovine plasma using a sensitive metabolite profiling method employing UPLC-QToF-MS and UPLC-QQQ-MS analysis. Over time, we have identified precurors of plant-based coumestans in lucerne and other legume crops and are now tracking key metabolites of abundance in bovine plasma over time in replicated feeding trials. We are also correlating these results with reproductive function.
1.Reed K; Agriculture 2016, 6(3), 35.
2.Ferreira-Dias, et al.; Theriogenology 2013, 80, 684-692.
3.Wyse, et al.; Metabolites 2021, 11(8), 550.
| Original language | English |
|---|---|
| Number of pages | 1 |
| Publication status | Published - 24 Nov 2023 |
| Event | 2023 RACI Natural Products Chemistry Group Annual One-Day Symposium - Charles Sturt University, Wagga Wagga, Australia Duration: 24 Nov 2023 → 24 Nov 2023 https://web.archive.org/web/20231108145056/https://raci.org.au/raci/web/event_display.aspx?EventKey=NNG1584 |
Conference
| Conference | 2023 RACI Natural Products Chemistry Group Annual One-Day Symposium |
|---|---|
| Country/Territory | Australia |
| City | Wagga Wagga |
| Period | 24/11/23 → 24/11/23 |
| Other | The RACI Natural Products Chemistry Group One-Day Symposium is an annual event that brings together researchers from all the sub-disciplines involved in the chemistry of natural products, including marine, microbial and terrestrial natural product isolation and characterisation, biosynthesis, total synthesis, pharmacology and pharmacognosy. Complete abstract book attached to PID 453431959 |
| Internet address |