Recombinant BFDV capsid protein

the 'I can't believe it's not butter' of viruses.

PL. Shearer, N. Bonne, B. Khalesi, ME. Stewart, M. Sharp, Shane Raidal

Research output: Book chapter/Published conference paperConference paper

Abstract

Psittacine beak and feather disease (PBFD) remains one of the most common viral diseases of both wild and captive birds, even after over 20 years of research and many attempts to develop options for vaccination and treatment. Many different researchers have attempted unsuccessfully to grow the virus in cell culture and a vaccine has even been produced, but is not a commercial reality due to the difficulty of producing enough antigen. Recombinant protein technology is widely used to study the structure and function of many proteins and these recombinant proteins are also gaining favour as alternatives to attenuated or inactivated vaccines. We present here the production of a recombinant His-tagged Beak and Feather Disease Virus (BFDV) capsid fusion protein using a baculovirus expression system and subsequent evidence that this protein is morphologically and functionally similar to native virus particles.
Original languageEnglish
Title of host publicationAAVAC and UEPV Annual Conference
EditorsGarry Cross
Place of PublicationAustralia
PublisherAAVAC
Pages187-190
Number of pages4
Publication statusPublished - 2006
EventAssociation of Avian Veterinarians Australasian Chapter (AAVAC) and Unusual and Exotic Pet Veterinarians (UEPV), Combined Conference - Wellington, New Zealand, New Zealand
Duration: 02 Sep 200606 Sep 2006

Conference

ConferenceAssociation of Avian Veterinarians Australasian Chapter (AAVAC) and Unusual and Exotic Pet Veterinarians (UEPV), Combined Conference
CountryNew Zealand
Period02/09/0606/09/06

Fingerprint

Circovirus
Butter
Capsid Proteins
Recombinant Proteins
Psittaciformes
Viral Fusion Proteins
Beak
Viruses
Feathers
Attenuated Vaccines
Inactivated Vaccines
Baculoviridae
Virus Diseases
Virion
Birds
Vaccination
Proteins
Vaccines
Cell Culture Techniques
Research Personnel

Cite this

Shearer, PL., Bonne, N., Khalesi, B., Stewart, ME., Sharp, M., & Raidal, S. (2006). Recombinant BFDV capsid protein: the 'I can't believe it's not butter' of viruses. In G. Cross (Ed.), AAVAC and UEPV Annual Conference (pp. 187-190). Australia: AAVAC.
Shearer, PL. ; Bonne, N. ; Khalesi, B. ; Stewart, ME. ; Sharp, M. ; Raidal, Shane. / Recombinant BFDV capsid protein : the 'I can't believe it's not butter' of viruses. AAVAC and UEPV Annual Conference. editor / Garry Cross. Australia : AAVAC, 2006. pp. 187-190
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abstract = "Psittacine beak and feather disease (PBFD) remains one of the most common viral diseases of both wild and captive birds, even after over 20 years of research and many attempts to develop options for vaccination and treatment. Many different researchers have attempted unsuccessfully to grow the virus in cell culture and a vaccine has even been produced, but is not a commercial reality due to the difficulty of producing enough antigen. Recombinant protein technology is widely used to study the structure and function of many proteins and these recombinant proteins are also gaining favour as alternatives to attenuated or inactivated vaccines. We present here the production of a recombinant His-tagged Beak and Feather Disease Virus (BFDV) capsid fusion protein using a baculovirus expression system and subsequent evidence that this protein is morphologically and functionally similar to native virus particles.",
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Shearer, PL, Bonne, N, Khalesi, B, Stewart, ME, Sharp, M & Raidal, S 2006, Recombinant BFDV capsid protein: the 'I can't believe it's not butter' of viruses. in G Cross (ed.), AAVAC and UEPV Annual Conference. AAVAC, Australia, pp. 187-190, Association of Avian Veterinarians Australasian Chapter (AAVAC) and Unusual and Exotic Pet Veterinarians (UEPV), Combined Conference, New Zealand, 02/09/06.

Recombinant BFDV capsid protein : the 'I can't believe it's not butter' of viruses. / Shearer, PL.; Bonne, N.; Khalesi, B.; Stewart, ME.; Sharp, M.; Raidal, Shane.

AAVAC and UEPV Annual Conference. ed. / Garry Cross. Australia : AAVAC, 2006. p. 187-190.

Research output: Book chapter/Published conference paperConference paper

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Shearer PL, Bonne N, Khalesi B, Stewart ME, Sharp M, Raidal S. Recombinant BFDV capsid protein: the 'I can't believe it's not butter' of viruses. In Cross G, editor, AAVAC and UEPV Annual Conference. Australia: AAVAC. 2006. p. 187-190