Regulation of adipogenesis and key adipogenic gene expression by nutritional molecules in 3T3 –L1 preadipocytes

Shuhan Ji, Pallavi Cheguru, Suresh Acharya, R.A. Hill

Research output: Contribution to journalMeeting Abstractpeer-review

Abstract

To investigate the function of nutrient molecules (1, 25-dihydroxyvitamin D (1, 25-(0H)z03) and retinoic acid (RA)) in regulation of adipogenesis, adipocyte differentiation and key adipogenic gene expression were studied in 3T3-L 1 preadipocytes. Lipid accumulation was measured by Oil Red 0 staining and expression of key adipogenic genes was quantified using quantitative real-time PCR. Adipogenic responses to 1, 25-(0HhD3 or RA were determined on days 2-10 after induction of adipogenesis with the traditional hormonal cocktail ± 1, 25-(0HhD3 or RA. In response to l, 25-(0HhD3 Cl 0-7, 1 O-s, 10-9 M), lipid accumulation and the expression of PPARy, C/EBPcx, FABP4 and SCD-1 were inhibited through day 10. In response to RA (1 0-6, 1 0-7 M), lipid accumulation and the expression of these genes were inhibited through day 8, rebounding to control levels by day 10. The greatest inhibitory effect was upon expression of FABP4. However, expression of SREBP-1 c was not affected. The lowest concentrations of 1, 25-(0H)zD3 or RA tested did not affect adipocyte
differentiation or expression of adipogenic genes. These results indicate that 1, 25-(0H)zD3 and RA inhibited adipogenesis via suppressing adipogenic specific genes, especially FABP4, and suggest that the roles of these nutrient factors in regulating adipogenesis will be informed by further studies of adipogenic specific gene promoter activity.
Original languageEnglish
JournalFASEB Journal
Volume27
Issue numberS1
Publication statusPublished - 2013

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