Acyl-CoA Thioesterases (ACOTs) perform a wide range of cellular functions by catalysingthe thiolytic cleavage of activated fatty acyl-CoAs. Substrates of ACOTs include short tolong-chain acyl-CoAs as well as a range of methyl-branched, and dicarboxylic bile acid-CoAs (M. C. Hunt & Alexson, 2008). Expression of ACOTs have been detected in bothprokaryotes and eukaryotes with expression in higher organisms being detected in cytosol,mitochondria, peroxisomes and endoplasmic reticulum (J. Yamada, 2005).Within the ACOT enzyme family, one member in particular, ACOT7 has recently beenidentified as playing a role inflammation through the production of arachidonic acid (AA).It was recently proposed that ACOT7-mediated AA production may provide acomplementary source of AA to the well characterised phospholipase A2 (PLA2) pathway(Satoru Sakuma, Usa, & Fujimoto, 2006); see Table 1 for review of Acot substrate specificity.Evidence for these observations was through experimental data showing that ACOT7possessed high substrate specificity for AA-CoA; the gene encoding ACOT7 was highlyexpressed in macrophages and up-regulated when stimulated by lipopolysaccharide (LPS);and that over-expression of the enzyme lead to an increase in prostaglandin production.Together, these observations highlight a novel role of ACOT7 in inflammation through theproduction of arachidonic acid from the thiolytic cleavage of activated polyunsaturatedomega-6 fatty acid C20:4-CoA (Forwood et al., 2007).
|Title of host publication||Inflammatory Diseases|
|Subtitle of host publication||A Modern Perspective|
|Number of pages||16|
|Publication status||Published - 2011|