Role of serum proteins in the cellular uptake of gold-peptide nanoconjugates

Guankui Wang, Madhusudhan R. Papasani, Patrick J. Hrdlicka, Rodney A. Hill

    Research output: Book chapter/Published conference paperConference paperpeer-review

    1 Citation (Scopus)

    Abstract

    Two different cysteine-terminated peptides, peptide A and peptide B, were successfully used to form nanoconjugates with gold nanoparticles (20 nm). We investigated the interactions of serum proteins with gold-peptide nanoconjugates and the cellular uptake of the nanoconjugates in the presence and absence of serum proteins. Electrophoresis gel data indicated the association of serum proteins with the nanoconjugates. In the presence and absence of serum in cell culture medium, both fluorophore-labeled peptide A and peptide B gold nanoconjugates were internalized by cells. However, free peptides show differential cell uptake: free peptide A being readily taken up, whereas free peptide B is at best only minimally taken up by cells. Following cell internalization, at least some nanoconjugates were dissociated, as free peptides were detected using fluorescence microscopy. These data suggest that Au NPs act as general nano-carriers. Serum proteins adsorbed to the nanoconjugates complicate the cellular mechanisms regulating their internalization.

    Original languageEnglish
    Title of host publicationNanotechnology 2010
    Subtitle of host publicationBio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
    Pages304-307
    Number of pages4
    Publication statusPublished - 09 Nov 2010
    EventNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, CA, United States
    Duration: 21 Jun 201024 Jun 2010

    Publication series

    NameNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
    Volume3

    Conference

    ConferenceNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
    Country/TerritoryUnited States
    CityAnaheim, CA
    Period21/06/1024/06/10

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