Secondary Metabolites Associated with Weed Suppression in Novel Annual Pasture Legumes

Research output: Other contribution to conferencePresentation only

Abstract

Australian farmers and researchers have embraced the use of introduced annual pasture species originating from Mediterranean regions of Europe and northern Africa for pasture diversity and N fixation. Recent research has focussed on the use of novel annual legumes including Ornithopus spp. (French serradella and yellow serradella) and Biserrula pelecinus L. (biserrula) for controlling weeds in rotational cropping systems for sustainable agricultural production. Several mechanisms are associated with the ability of pasture legumes to suppress weeds including 1) competition for resources and 2) release of secondary metabolites into the rhizosphere1 through root exudation. Laboratory bioassays were therefore designed to evaluate the phytotoxic potential of fresh shoot extracts of pasture legumes using germinating seedlings of Lepidium sativum L. (garden cress) and Lolium rigidum Gaud. (annual ryegrass). The I50 of crude extracts was calculated for radicle elongation for both test species. Non-targeted and targeted metabolic profiling was performed using ultra-high pressure liquid chromatography (Agilent, 1200 Infinity) coupled with quadrupole-time of flight mass spectrometry (Agilent 6530). Multi-regression analysis of crude shoot extracts was performed to determine an association between phytotoxicity and the abundance of secondary metabolites. Key bioactive metabolites of interest were identified using a personal compound database library (PCDL) and known standards. Fresh extracts of biserrula (0.78 mg/mL respectively) and yellow serradella (0.84 mg/mL) exhibited the greatest phytotoxic activity against radicle elongation of garden cress and annual ryegrass seedlings respectively. Step-wise multiple regression analysis demonstrated a strong association of between bioactivity and the presence of five metabolites including quercetin and kaempferol and their associated glycosides2. A significantly higher relative abundance of key phytotoxic flavonoids and their glycosides in the rhizosphere of field – collected soil samples further confirmed the phytotoxic potential of biserrula and yellow serradella.
Original languageEnglish
Publication statusPublished - 28 Sep 2018
Event2018 RACI Natural Products Chemistry one-day Symposium - University of New South Wales, Sydney, Australia
Duration: 28 Sep 201828 Sep 2018
https://www.raci.org.au/branches/nsw-branch/natural-products-chemistry

Conference

Conference2018 RACI Natural Products Chemistry one-day Symposium
CountryAustralia
CitySydney
Period28/09/1828/09/18
Internet address

Fingerprint

Ornithopus compressus
forage legumes
Lepidium sativum
secondary metabolites
weed control
root radicle
extracts
Lolium
Ornithopus
regression analysis
Ornithopus sativus
weeds
metabolites
Lolium rigidum
shoots
seedlings
exudation
metabolomics
Northern Africa
kaempferol

Cite this

Latif, S., Gurusinghe, S., Weston, P., Quinn, J., & Weston, L. A. (2018). Secondary Metabolites Associated with Weed Suppression in Novel Annual Pasture Legumes. Paper presented at 2018 RACI Natural Products Chemistry one-day Symposium, Sydney, Australia.
@conference{9996f1268e8542e4a6447cd9d6df9557,
title = "Secondary Metabolites Associated with Weed Suppression in Novel Annual Pasture Legumes",
abstract = "Australian farmers and researchers have embraced the use of introduced annual pasture species originating from Mediterranean regions of Europe and northern Africa for pasture diversity and N fixation. Recent research has focussed on the use of novel annual legumes including Ornithopus spp. (French serradella and yellow serradella) and Biserrula pelecinus L. (biserrula) for controlling weeds in rotational cropping systems for sustainable agricultural production. Several mechanisms are associated with the ability of pasture legumes to suppress weeds including 1) competition for resources and 2) release of secondary metabolites into the rhizosphere1 through root exudation. Laboratory bioassays were therefore designed to evaluate the phytotoxic potential of fresh shoot extracts of pasture legumes using germinating seedlings of Lepidium sativum L. (garden cress) and Lolium rigidum Gaud. (annual ryegrass). The I50 of crude extracts was calculated for radicle elongation for both test species. Non-targeted and targeted metabolic profiling was performed using ultra-high pressure liquid chromatography (Agilent, 1200 Infinity) coupled with quadrupole-time of flight mass spectrometry (Agilent 6530). Multi-regression analysis of crude shoot extracts was performed to determine an association between phytotoxicity and the abundance of secondary metabolites. Key bioactive metabolites of interest were identified using a personal compound database library (PCDL) and known standards. Fresh extracts of biserrula (0.78 mg/mL respectively) and yellow serradella (0.84 mg/mL) exhibited the greatest phytotoxic activity against radicle elongation of garden cress and annual ryegrass seedlings respectively. Step-wise multiple regression analysis demonstrated a strong association of between bioactivity and the presence of five metabolites including quercetin and kaempferol and their associated glycosides2. A significantly higher relative abundance of key phytotoxic flavonoids and their glycosides in the rhizosphere of field – collected soil samples further confirmed the phytotoxic potential of biserrula and yellow serradella.",
author = "Sajid Latif and Saliya Gurusinghe and Paul Weston and Jane Quinn and Weston, {Leslie A.}",
year = "2018",
month = "9",
day = "28",
language = "English",
note = "2018 RACI Natural Products Chemistry one-day Symposium ; Conference date: 28-09-2018 Through 28-09-2018",
url = "https://www.raci.org.au/branches/nsw-branch/natural-products-chemistry",

}

Latif, S, Gurusinghe, S, Weston, P, Quinn, J & Weston, LA 2018, 'Secondary Metabolites Associated with Weed Suppression in Novel Annual Pasture Legumes', Paper presented at 2018 RACI Natural Products Chemistry one-day Symposium, Sydney, Australia, 28/09/18 - 28/09/18.

Secondary Metabolites Associated with Weed Suppression in Novel Annual Pasture Legumes. / Latif, Sajid; Gurusinghe, Saliya; Weston, Paul; Quinn, Jane; Weston, Leslie A.

2018. Paper presented at 2018 RACI Natural Products Chemistry one-day Symposium, Sydney, Australia.

Research output: Other contribution to conferencePresentation only

TY - CONF

T1 - Secondary Metabolites Associated with Weed Suppression in Novel Annual Pasture Legumes

AU - Latif, Sajid

AU - Gurusinghe, Saliya

AU - Weston, Paul

AU - Quinn, Jane

AU - Weston, Leslie A.

PY - 2018/9/28

Y1 - 2018/9/28

N2 - Australian farmers and researchers have embraced the use of introduced annual pasture species originating from Mediterranean regions of Europe and northern Africa for pasture diversity and N fixation. Recent research has focussed on the use of novel annual legumes including Ornithopus spp. (French serradella and yellow serradella) and Biserrula pelecinus L. (biserrula) for controlling weeds in rotational cropping systems for sustainable agricultural production. Several mechanisms are associated with the ability of pasture legumes to suppress weeds including 1) competition for resources and 2) release of secondary metabolites into the rhizosphere1 through root exudation. Laboratory bioassays were therefore designed to evaluate the phytotoxic potential of fresh shoot extracts of pasture legumes using germinating seedlings of Lepidium sativum L. (garden cress) and Lolium rigidum Gaud. (annual ryegrass). The I50 of crude extracts was calculated for radicle elongation for both test species. Non-targeted and targeted metabolic profiling was performed using ultra-high pressure liquid chromatography (Agilent, 1200 Infinity) coupled with quadrupole-time of flight mass spectrometry (Agilent 6530). Multi-regression analysis of crude shoot extracts was performed to determine an association between phytotoxicity and the abundance of secondary metabolites. Key bioactive metabolites of interest were identified using a personal compound database library (PCDL) and known standards. Fresh extracts of biserrula (0.78 mg/mL respectively) and yellow serradella (0.84 mg/mL) exhibited the greatest phytotoxic activity against radicle elongation of garden cress and annual ryegrass seedlings respectively. Step-wise multiple regression analysis demonstrated a strong association of between bioactivity and the presence of five metabolites including quercetin and kaempferol and their associated glycosides2. A significantly higher relative abundance of key phytotoxic flavonoids and their glycosides in the rhizosphere of field – collected soil samples further confirmed the phytotoxic potential of biserrula and yellow serradella.

AB - Australian farmers and researchers have embraced the use of introduced annual pasture species originating from Mediterranean regions of Europe and northern Africa for pasture diversity and N fixation. Recent research has focussed on the use of novel annual legumes including Ornithopus spp. (French serradella and yellow serradella) and Biserrula pelecinus L. (biserrula) for controlling weeds in rotational cropping systems for sustainable agricultural production. Several mechanisms are associated with the ability of pasture legumes to suppress weeds including 1) competition for resources and 2) release of secondary metabolites into the rhizosphere1 through root exudation. Laboratory bioassays were therefore designed to evaluate the phytotoxic potential of fresh shoot extracts of pasture legumes using germinating seedlings of Lepidium sativum L. (garden cress) and Lolium rigidum Gaud. (annual ryegrass). The I50 of crude extracts was calculated for radicle elongation for both test species. Non-targeted and targeted metabolic profiling was performed using ultra-high pressure liquid chromatography (Agilent, 1200 Infinity) coupled with quadrupole-time of flight mass spectrometry (Agilent 6530). Multi-regression analysis of crude shoot extracts was performed to determine an association between phytotoxicity and the abundance of secondary metabolites. Key bioactive metabolites of interest were identified using a personal compound database library (PCDL) and known standards. Fresh extracts of biserrula (0.78 mg/mL respectively) and yellow serradella (0.84 mg/mL) exhibited the greatest phytotoxic activity against radicle elongation of garden cress and annual ryegrass seedlings respectively. Step-wise multiple regression analysis demonstrated a strong association of between bioactivity and the presence of five metabolites including quercetin and kaempferol and their associated glycosides2. A significantly higher relative abundance of key phytotoxic flavonoids and their glycosides in the rhizosphere of field – collected soil samples further confirmed the phytotoxic potential of biserrula and yellow serradella.

M3 - Presentation only

ER -

Latif S, Gurusinghe S, Weston P, Quinn J, Weston LA. Secondary Metabolites Associated with Weed Suppression in Novel Annual Pasture Legumes. 2018. Paper presented at 2018 RACI Natural Products Chemistry one-day Symposium, Sydney, Australia.