Sensitivity and specificity of HA, HI and PCR for detecting psittacine beak and feather disease virus (BFDV) testing.

Shane Raidal, B Khalesi, N Bonne, M. Stewart, M. Sharp

Research output: Book chapter/Published conference paperConference paper

Abstract

Psittacine beak and feather disease (PBFD) continues to be a major cause of ill health in companion and wild birds throughout Australia and is listed as a key threatening process for several endangered psittacine bird species. The virus that causes the disease (BFDV) is a haemagglutinating circovirus which has permitted the development of haemagglutination (HA) and haemagglutination inhibition (HI) assays for the virus and antibody responses to infection, respectively. These assays, as well as PCR testing based on the relatively conserved ORF-V1, are in wide use throughout Australia and the world for diagnosing infection. We decided to determine the limit of detection of our HA and PCR assays and compared the three tests on feather and blood samples sent to us for routine diagnostic testing.
Original languageEnglish
Title of host publicationUnusual and exotic pet veterinarians
Subtitle of host publicationAAVAC and UEPV 2006 annual conference proceedings
EditorsGarry Cross
Place of PublicationPalmerston North
PublisherAustralian Committee Association of Avian Veterinarians
Pages103-112
Number of pages10
Publication statusPublished - 2006
EventAustralian Committee Association of Avian Veterinarians Annual Conference, - Wellington, New Zealand, New Zealand
Duration: 02 Sep 200606 Sep 2006

Conference

ConferenceAustralian Committee Association of Avian Veterinarians Annual Conference,
CountryNew Zealand
Period02/09/0606/09/06

Fingerprint

Beak and feather disease virus
hemagglutination
feathers
Circovirus
viruses
hemagglutination inhibition test
beak
testing
wild birds
assays
infection
detection limit
antibodies
birds
blood
sampling

Cite this

Raidal, S., Khalesi, B., Bonne, N., Stewart, M., & Sharp, M. (2006). Sensitivity and specificity of HA, HI and PCR for detecting psittacine beak and feather disease virus (BFDV) testing. In G. Cross (Ed.), Unusual and exotic pet veterinarians: AAVAC and UEPV 2006 annual conference proceedings (pp. 103-112). Palmerston North: Australian Committee Association of Avian Veterinarians.
Raidal, Shane ; Khalesi, B ; Bonne, N ; Stewart, M. ; Sharp, M. / Sensitivity and specificity of HA, HI and PCR for detecting psittacine beak and feather disease virus (BFDV) testing. Unusual and exotic pet veterinarians: AAVAC and UEPV 2006 annual conference proceedings. editor / Garry Cross. Palmerston North : Australian Committee Association of Avian Veterinarians, 2006. pp. 103-112
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abstract = "Psittacine beak and feather disease (PBFD) continues to be a major cause of ill health in companion and wild birds throughout Australia and is listed as a key threatening process for several endangered psittacine bird species. The virus that causes the disease (BFDV) is a haemagglutinating circovirus which has permitted the development of haemagglutination (HA) and haemagglutination inhibition (HI) assays for the virus and antibody responses to infection, respectively. These assays, as well as PCR testing based on the relatively conserved ORF-V1, are in wide use throughout Australia and the world for diagnosing infection. We decided to determine the limit of detection of our HA and PCR assays and compared the three tests on feather and blood samples sent to us for routine diagnostic testing.",
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Raidal, S, Khalesi, B, Bonne, N, Stewart, M & Sharp, M 2006, Sensitivity and specificity of HA, HI and PCR for detecting psittacine beak and feather disease virus (BFDV) testing. in G Cross (ed.), Unusual and exotic pet veterinarians: AAVAC and UEPV 2006 annual conference proceedings. Australian Committee Association of Avian Veterinarians, Palmerston North, pp. 103-112, Australian Committee Association of Avian Veterinarians Annual Conference, New Zealand, 02/09/06.

Sensitivity and specificity of HA, HI and PCR for detecting psittacine beak and feather disease virus (BFDV) testing. / Raidal, Shane; Khalesi, B; Bonne, N; Stewart, M.; Sharp, M.

Unusual and exotic pet veterinarians: AAVAC and UEPV 2006 annual conference proceedings. ed. / Garry Cross. Palmerston North : Australian Committee Association of Avian Veterinarians, 2006. p. 103-112.

Research output: Book chapter/Published conference paperConference paper

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AB - Psittacine beak and feather disease (PBFD) continues to be a major cause of ill health in companion and wild birds throughout Australia and is listed as a key threatening process for several endangered psittacine bird species. The virus that causes the disease (BFDV) is a haemagglutinating circovirus which has permitted the development of haemagglutination (HA) and haemagglutination inhibition (HI) assays for the virus and antibody responses to infection, respectively. These assays, as well as PCR testing based on the relatively conserved ORF-V1, are in wide use throughout Australia and the world for diagnosing infection. We decided to determine the limit of detection of our HA and PCR assays and compared the three tests on feather and blood samples sent to us for routine diagnostic testing.

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Raidal S, Khalesi B, Bonne N, Stewart M, Sharp M. Sensitivity and specificity of HA, HI and PCR for detecting psittacine beak and feather disease virus (BFDV) testing. In Cross G, editor, Unusual and exotic pet veterinarians: AAVAC and UEPV 2006 annual conference proceedings. Palmerston North: Australian Committee Association of Avian Veterinarians. 2006. p. 103-112