Sonic hedgehog signaling promotes growth of oral squamous cell carcinoma cells associated with bone destruction

Tatsuki Honami; Tsuyoshi Shimo; Tatsuo Okui; Naito Kurio; Nur Hassan; Masahiro Iwamoto; Akira Sasaki

doi:10.1016/j.oraloncology.2011.08.026

Sonic hedgehog signaling promotes growth of oral squamous cell carcinoma cells associated with bone destruction

Tatsuki Honami, Tsuyoshi Shimo, Tatsuo Okui, Naito Kurio, Nur Hassan, Masahiro Iwamoto, Akira Sasaki

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Abstract

Sonic hedgehog (Shh) and its signaling have been identified in several human cancers, and increased levels of its expression appear to correlate with disease progression and metastasis. However, the role
of Shh in bone destruction associated with oral squamous cell carcinomas, which frequently invade the maxilla or the mandible, is still unclear. In this study we show that the use of siRNA for Shh to block SHH secreted by SAS oral squamous cell carcinoma cells suppressed the tumor growth and tumor angiogenesis of subcutaneous SAS xenografts in vivo. Moreover, blockade of Shh in SAS cells decreased tumor growth and osteoclast number in a tibial metaphysis mouse model. Signifi-
cantly, we clearly show that SHH stimulated osteoclast formation in a co-culture system consisting of murine bone stromal ST2 cells and murine CD11b+ bone marrow cells. These findings suggest that
Shh signaling is a potential target for the treatment of oral squamous cell carcinoma associated with bone destruction.

Original language	English
Pages (from-to)	49-55
Number of pages	7
Journal	Oral Oncology
Volume	48
Issue number	1
Early online date	Sept 2011
DOIs	https://doi.org/10.1016/j.oraloncology.2011.08.026
Publication status	Published - 2012

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10.1016/j.oraloncology.2011.08.026

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title = "Sonic hedgehog signaling promotes growth of oral squamous cell carcinoma cells associated with bone destruction",

abstract = "Sonic hedgehog (Shh) and its signaling have been identified in several human cancers, and increased levels of its expression appear to correlate with disease progression and metastasis. However, the roleof Shh in bone destruction associated with oral squamous cell carcinomas, which frequently invade the maxilla or the mandible, is still unclear. In this study we show that the use of siRNA for Shh to block SHH secreted by SAS oral squamous cell carcinoma cells suppressed the tumor growth and tumor angiogenesis of subcutaneous SAS xenografts in vivo. Moreover, blockade of Shh in SAS cells decreased tumor growth and osteoclast number in a tibial metaphysis mouse model. Signifi-cantly, we clearly show that SHH stimulated osteoclast formation in a co-culture system consisting of murine bone stromal ST2 cells and murine CD11b+ bone marrow cells. These findings suggest thatShh signaling is a potential target for the treatment of oral squamous cell carcinoma associated with bone destruction.",

author = "Tatsuki Honami and Tsuyoshi Shimo and Tatsuo Okui and Naito Kurio and Nur Hassan and Masahiro Iwamoto and Akira Sasaki",

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doi = "10.1016/j.oraloncology.2011.08.026",

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T1 - Sonic hedgehog signaling promotes growth of oral squamous cell carcinoma cells associated with bone destruction

AU - Honami, Tatsuki

AU - Shimo, Tsuyoshi

AU - Okui, Tatsuo

AU - Kurio, Naito

AU - Hassan, Nur

AU - Iwamoto, Masahiro

AU - Sasaki, Akira

PY - 2012

Y1 - 2012

N2 - Sonic hedgehog (Shh) and its signaling have been identified in several human cancers, and increased levels of its expression appear to correlate with disease progression and metastasis. However, the roleof Shh in bone destruction associated with oral squamous cell carcinomas, which frequently invade the maxilla or the mandible, is still unclear. In this study we show that the use of siRNA for Shh to block SHH secreted by SAS oral squamous cell carcinoma cells suppressed the tumor growth and tumor angiogenesis of subcutaneous SAS xenografts in vivo. Moreover, blockade of Shh in SAS cells decreased tumor growth and osteoclast number in a tibial metaphysis mouse model. Signifi-cantly, we clearly show that SHH stimulated osteoclast formation in a co-culture system consisting of murine bone stromal ST2 cells and murine CD11b+ bone marrow cells. These findings suggest thatShh signaling is a potential target for the treatment of oral squamous cell carcinoma associated with bone destruction.

AB - Sonic hedgehog (Shh) and its signaling have been identified in several human cancers, and increased levels of its expression appear to correlate with disease progression and metastasis. However, the roleof Shh in bone destruction associated with oral squamous cell carcinomas, which frequently invade the maxilla or the mandible, is still unclear. In this study we show that the use of siRNA for Shh to block SHH secreted by SAS oral squamous cell carcinoma cells suppressed the tumor growth and tumor angiogenesis of subcutaneous SAS xenografts in vivo. Moreover, blockade of Shh in SAS cells decreased tumor growth and osteoclast number in a tibial metaphysis mouse model. Signifi-cantly, we clearly show that SHH stimulated osteoclast formation in a co-culture system consisting of murine bone stromal ST2 cells and murine CD11b+ bone marrow cells. These findings suggest thatShh signaling is a potential target for the treatment of oral squamous cell carcinoma associated with bone destruction.

U2 - 10.1016/j.oraloncology.2011.08.026

DO - 10.1016/j.oraloncology.2011.08.026

M3 - Article

SN - 1368-8375

VL - 48

SP - 49

EP - 55

JO - Oral Oncology

JF - Oral Oncology

IS - 1

ER -

Sonic hedgehog signaling promotes growth of oral squamous cell carcinoma cells associated with bone destruction

Abstract

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