Structurally manipulated antioxidant peptides derived from wheat bran: Preparation and identification

Min Zhuang, Jiaqing Li, Anqi Wang, Gaoheng Li, Sheng Ke, Xuanyu Wang, Ming Ning, Zhanwu Sheng, Bing Wang, Bing Wang, Zhongkai Zhou

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Bioactive peptide's development is facing two challenges in terms of its lower yield and limited understanding of structurally orientated functionality. Therefore, peptides were prepared from wheat bran via a cocktail enzyme for achieving a higher level of hydrophobic amino acids than traditional method. The obtained peptides exhibited great antioxidant activities against H2O2-induced oxidative stress in HepG2 cells. Among them, 91 bioactive peptides were selected through the virtual screening, and their N-terminal and C-terminal contained many hydrophobic amino acids. Then the peptides with capacity to interact with Keap1 were identified by in silico simulation, because Keap1 acts as a sensor of redox insults. The results revealed that peptides DLDW and DLGL demonstrated the highest binding affinities, and a bridge was formed between Asp of DLGL and Arg415 of Klech domain, contributing to interfering Keap1-Nrf2 interaction. These findings implied a potential application of wheat bran peptides as nutraceuticals and health-promoting ingredients.

Original languageEnglish
Article number138465
JournalFood Chemistry
Publication statusPublished - 01 Jun 2024


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