Structurally manipulated antioxidant peptides derived from wheat bran: Preparation and identification

Min Zhuang, Jiaqing Li, Anqi Wang, Gaoheng Li, Sheng Ke, Xuanyu Wang, Ming Ning, Zhanwu Sheng, Bing Wang, Bing Wang, Zhongkai Zhou

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Bioactive peptide's development is facing two challenges in terms of its lower yield and limited understanding of structurally orientated functionality. Therefore, peptides were prepared from wheat bran via a cocktail enzyme for achieving a higher level of hydrophobic amino acids than traditional method. The obtained peptides exhibited great antioxidant activities against H2O2-induced oxidative stress in HepG2 cells. Among them, 91 bioactive peptides were selected through the virtual screening, and their N-terminal and C-terminal contained many hydrophobic amino acids. Then the peptides with capacity to interact with Keap1 were identified by in silico simulation, because Keap1 acts as a sensor of redox insults. The results revealed that peptides DLDW and DLGL demonstrated the highest binding affinities, and a bridge was formed between Asp of DLGL and Arg415 of Klech domain, contributing to interfering Keap1-Nrf2 interaction. These findings implied a potential application of wheat bran peptides as nutraceuticals and health-promoting ingredients.

Original languageEnglish
Article number138465
JournalFood Chemistry
Volume442
DOIs
Publication statusPublished - 01 Jun 2024

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