Superovulatory response and embryo quality in beef cattle grazing lucerne

Jessica Wyse; Rory Nevard; Jaymie Loy; Saliya Gurusinghe; Jeff McCormick; Leslie  Weston; Cyril Stephen

Abstract

Livestock with access to pastures with high phyto-oestrogen concentrations may experience compromised reproductive functions.1. This study was conducted to investigate the impact of phytooestrogens on superovulatory response and embryo quality in cattle grazing lucerne.
An eight-week grazing trial was conducted using 20 pluriparous Angus cows on lucerne and ryegrass pastures. Cows were subjected to an oestrus synchronisation programme using CIDR, oestradiol
benzoate and cloprostenol as previously described2
. Four days following CIDR insertion, FSH (Folltropin
V, 20 mg/mL) was injected twice daily (q12 hrs x 4 days), in a tapering dose, to superstimulate follicles.
On the last day of FSH injections, CIDR’s were removed and cloprostenol (500 μg) was injected twice
(q12 hrs). Gonadorelin (250 μg) was administered 24 hrs after removing the CIDR’s, and the cows were
artificially inseminated with frozen semen (AM and PM) from a proven sire the following day. Embryo
recovery and grading occurred seven days post insemination. Blood and pasture samples were collected
every seven days to measure phyto-oestrogens3
. Metabolomic profiling of pasture and plasma samples
was performed using HPLC-MS-QTOF and HPLC-MS-QQQ.
Numerous phyto-oestrogens including, coumestrol, were found in both the plasma and pasture
samples. Significant embryo quality differences were noted between cattle grazing lucerne for eight
weeks and the control group. There was no effect on superovulatory response in both groups.
Our findings suggest that phyto-oestrogens, although not directly influencing the superovulatory
response, can influence the embryo quality in cattle grazing lucerne for greater than three weeks,
particularly where concentrations exceed 25 mg/kg DM.

References:
1. Borzym E, Bobowiec R, Kosior-Korzecka U, Martelli F, Burmaczuk A. Disturbances of cow oocyte
maturation by phytoestrogens. Med. Weter. 2008;64:1107–1111.
2. Bó GA, Mapletoft RJ. Historical perspectives and recent research on superovulation in cattle.
Theriogenology. 2014 Jan 1;81(1):38-48.
3. Wyse J, Latif S, Gurusinghe S, Berntsen ED, Weston LA, Stephen C. Characterization of
phytoestrogens in Medicago sativa L. and grazing beef cattle. Metabolites. 2021;11

Original language	English
Number of pages	1
Publication status	Published - 2023
Event	ANZCVS Science Week 2023: Animal Reproduction & Cattle Chapter - Gold Coast Convention and Exhibition Centre, Gold Coast, Australia Duration: 27 Jul 2023 → 29 Jul 2023 https://web.archive.org/web/20230326062527/https://vetscienceweek.com.au/ (WayBack Machine link to conference website)

Conference

Conference	ANZCVS Science Week 2023
Country/Territory	Australia
City	Gold Coast
Period	27/07/23 → 29/07/23
Other	On behalf of the Australian and New Zealand College of Veterinary Scientists (ANZCVS) Council and Science Week Organising Committee, I welcome you to the 21st annual Science Week Conference to be held from 27- 29 July 2023. The continued appeal of College Science Week to delegates and industry partners alike has allowed the College to secure the Gold Coast Convention and Exhibition Centre as the venue for the next two years. College Science Week aims to gather global experts from the veterinary field to present, discuss and explore cutting-edge topics in veterinary science and research. Sessions present the latest research innovations and advanced topic reviews in a diverse range of veterinary fields. It is also a forum that welcomes and supports early career researchers, such as those undertaking internships or residency training programs. Delegates will enjoy furthering their knowledge, interacting with industry partners and socialising with colleagues. The scientific program will extend over 3 days, but pre-conference workshops are also being hosted by several Chapters. The trade exhibition area will be more extensive than previous years, and the industry representatives are always excited to showcase how they can improve your veterinary work with their services, products, and programs. Finally, there will be ample opportunity and space to connect and reconnect with colleagues socially, be it over Chapter breakfasts, Alumni Dinners or the Friday night Conference Dinner which is certain to be a highlight of the conference. I look forward to seeing you all in July 2023.
Internet address	https://web.archive.org/web/20230326062527/https://vetscienceweek.com.au/ (WayBack Machine link to conference website)

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SW23_Research-Abstract-WYSEFinal published version, 117 KB

1 Doctoral Thesis

Characterisation of Coumestans in Lucerne and Their Effect on Fertility in Beef Cattle
Wyse, J., 2025, Australia: Charles Sturt University. 207 p.
Research output: Thesis › Doctoral Thesis

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title = "Superovulatory response and embryo quality in beef cattle grazing lucerne",

abstract = "Livestock with access to pastures with high phyto-oestrogen concentrations may experience compromised reproductive functions.1. This study was conducted to investigate the impact of phytooestrogens on superovulatory response and embryo quality in cattle grazing lucerne. An eight-week grazing trial was conducted using 20 pluriparous Angus cows on lucerne and ryegrass pastures. Cows were subjected to an oestrus synchronisation programme using CIDR, oestradiol benzoate and cloprostenol as previously described2 . Four days following CIDR insertion, FSH (Folltropin V, 20 mg/mL) was injected twice daily (q12 hrs x 4 days), in a tapering dose, to superstimulate follicles. On the last day of FSH injections, CIDR{\textquoteright}s were removed and cloprostenol (500 μg) was injected twice (q12 hrs). Gonadorelin (250 μg) was administered 24 hrs after removing the CIDR{\textquoteright}s, and the cows were artificially inseminated with frozen semen (AM and PM) from a proven sire the following day. Embryo recovery and grading occurred seven days post insemination. Blood and pasture samples were collected every seven days to measure phyto-oestrogens3 . Metabolomic profiling of pasture and plasma samples was performed using HPLC-MS-QTOF and HPLC-MS-QQQ. Numerous phyto-oestrogens including, coumestrol, were found in both the plasma and pasture samples. Significant embryo quality differences were noted between cattle grazing lucerne for eight weeks and the control group. There was no effect on superovulatory response in both groups. Our findings suggest that phyto-oestrogens, although not directly influencing the superovulatory response, can influence the embryo quality in cattle grazing lucerne for greater than three weeks, particularly where concentrations exceed 25 mg/kg DM. References: 1. Borzym E, Bobowiec R, Kosior-Korzecka U, Martelli F, Burmaczuk A. Disturbances of cow oocyte maturation by phytoestrogens. Med. Weter. 2008;64:1107–1111. 2. B{\'o} GA, Mapletoft RJ. Historical perspectives and recent research on superovulation in cattle. Theriogenology. 2014 Jan 1;81(1):38-48. 3. Wyse J, Latif S, Gurusinghe S, Berntsen ED, Weston LA, Stephen C. Characterization of phytoestrogens in Medicago sativa L. and grazing beef cattle. Metabolites. 2021;11",

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T1 - Superovulatory response and embryo quality in beef cattle grazing lucerne

AU - Nevard, Rory

AU - Loy, Jaymie

AU - Gurusinghe, Saliya

AU - McCormick, Jeff

AU - Weston, Leslie

AU - Stephen, Cyril

A2 - Wyse, Jessica

PY - 2023

Y1 - 2023

N2 - Livestock with access to pastures with high phyto-oestrogen concentrations may experience compromised reproductive functions.1. This study was conducted to investigate the impact of phytooestrogens on superovulatory response and embryo quality in cattle grazing lucerne. An eight-week grazing trial was conducted using 20 pluriparous Angus cows on lucerne and ryegrass pastures. Cows were subjected to an oestrus synchronisation programme using CIDR, oestradiol benzoate and cloprostenol as previously described2 . Four days following CIDR insertion, FSH (Folltropin V, 20 mg/mL) was injected twice daily (q12 hrs x 4 days), in a tapering dose, to superstimulate follicles. On the last day of FSH injections, CIDR’s were removed and cloprostenol (500 μg) was injected twice (q12 hrs). Gonadorelin (250 μg) was administered 24 hrs after removing the CIDR’s, and the cows were artificially inseminated with frozen semen (AM and PM) from a proven sire the following day. Embryo recovery and grading occurred seven days post insemination. Blood and pasture samples were collected every seven days to measure phyto-oestrogens3 . Metabolomic profiling of pasture and plasma samples was performed using HPLC-MS-QTOF and HPLC-MS-QQQ. Numerous phyto-oestrogens including, coumestrol, were found in both the plasma and pasture samples. Significant embryo quality differences were noted between cattle grazing lucerne for eight weeks and the control group. There was no effect on superovulatory response in both groups. Our findings suggest that phyto-oestrogens, although not directly influencing the superovulatory response, can influence the embryo quality in cattle grazing lucerne for greater than three weeks, particularly where concentrations exceed 25 mg/kg DM. References: 1. Borzym E, Bobowiec R, Kosior-Korzecka U, Martelli F, Burmaczuk A. Disturbances of cow oocyte maturation by phytoestrogens. Med. Weter. 2008;64:1107–1111. 2. Bó GA, Mapletoft RJ. Historical perspectives and recent research on superovulation in cattle. Theriogenology. 2014 Jan 1;81(1):38-48. 3. Wyse J, Latif S, Gurusinghe S, Berntsen ED, Weston LA, Stephen C. Characterization of phytoestrogens in Medicago sativa L. and grazing beef cattle. Metabolites. 2021;11

AB - Livestock with access to pastures with high phyto-oestrogen concentrations may experience compromised reproductive functions.1. This study was conducted to investigate the impact of phytooestrogens on superovulatory response and embryo quality in cattle grazing lucerne. An eight-week grazing trial was conducted using 20 pluriparous Angus cows on lucerne and ryegrass pastures. Cows were subjected to an oestrus synchronisation programme using CIDR, oestradiol benzoate and cloprostenol as previously described2 . Four days following CIDR insertion, FSH (Folltropin V, 20 mg/mL) was injected twice daily (q12 hrs x 4 days), in a tapering dose, to superstimulate follicles. On the last day of FSH injections, CIDR’s were removed and cloprostenol (500 μg) was injected twice (q12 hrs). Gonadorelin (250 μg) was administered 24 hrs after removing the CIDR’s, and the cows were artificially inseminated with frozen semen (AM and PM) from a proven sire the following day. Embryo recovery and grading occurred seven days post insemination. Blood and pasture samples were collected every seven days to measure phyto-oestrogens3 . Metabolomic profiling of pasture and plasma samples was performed using HPLC-MS-QTOF and HPLC-MS-QQQ. Numerous phyto-oestrogens including, coumestrol, were found in both the plasma and pasture samples. Significant embryo quality differences were noted between cattle grazing lucerne for eight weeks and the control group. There was no effect on superovulatory response in both groups. Our findings suggest that phyto-oestrogens, although not directly influencing the superovulatory response, can influence the embryo quality in cattle grazing lucerne for greater than three weeks, particularly where concentrations exceed 25 mg/kg DM. References: 1. Borzym E, Bobowiec R, Kosior-Korzecka U, Martelli F, Burmaczuk A. Disturbances of cow oocyte maturation by phytoestrogens. Med. Weter. 2008;64:1107–1111. 2. Bó GA, Mapletoft RJ. Historical perspectives and recent research on superovulation in cattle. Theriogenology. 2014 Jan 1;81(1):38-48. 3. Wyse J, Latif S, Gurusinghe S, Berntsen ED, Weston LA, Stephen C. Characterization of phytoestrogens in Medicago sativa L. and grazing beef cattle. Metabolites. 2021;11

KW - Phytoestrogens

KW - Bovine reproduction

M3 - Abstract

T2 - ANZCVS Science Week 2023

Y2 - 27 July 2023 through 29 July 2023

ER -

Superovulatory response and embryo quality in beef cattle grazing lucerne

Abstract

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Research output

Characterisation of Coumestans in Lucerne and Their Effect on Fertility in Beef Cattle

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