Temporal changes in gastrointestinal microbiomes of beef cattle on feedlot placement

Research output: Other contribution to conferenceAbstract

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Abstract

Introduction: A majority of beef cattle are finished in feedlots after purchase. However, feedlot placement has also been found to adversely influence cattle health, predisposing them to many diseases including Bovine Respiratory Disease
(BRD). BRD is an important feedlot disease causing significant economic losses in beef cattle. While the influence of feedlot transition on disease predisposition is known to be mechanistically complex and multifactorial, the host microbiome could play a key role. Accordingly, the current project aimed to characterise temporal changes in gastrointestinal microbiota of beef cattle upon feedlot placement.
Methods: Faecal samples from 30 cattle were sampled upon feedlot induction (day 0), and then repeat sampling was carried out on days 2, 7 and 14. Lung scores were obtained on the last day of sampling (day 14) and subsequently
15 samples were chosen based on consistency of faecal samples across all four days of sampling, and lung scores obtained on day 14. Total DNA extracted from these faecal samples were subjected to 16s rDNA sequencing to determine the relative abundance of microbial species.
Results: Results indicate that feedlot transition significantly impact gastrointestinal microbiome profiles, particularly around 2 days after feedlot placement. On day 2, the proportion of key groups of bacteria previously associated with gastrointestinal diseases, like proteobacteria and actinobacteria, were significantly affected.
Conclusions: Overall, results suggest that gastrointestinal microbiota are significantly impacted due to feedlot placement of beef cattle, and their role in contributing to feedlot associated disease predisposition, including BRD, warrants
further investigated
Original languageEnglish
Pages134
Number of pages1
Publication statusPublished - 21 Aug 2019
Event2019 Northern Beef Research Update Conference: Bush to bright lights - Brisbane Convention & Exhibition Centre, Brisbane, Australia
Duration: 19 Aug 201922 Aug 2019
https://www.nbruc2019.com/
https://researchoutput.csu.edu.au/admin/files/32129155/NBRUC_Conference_program.pdf (program and abstracts)

Conference

Conference2019 Northern Beef Research Update Conference
Abbreviated titleBeef Research
CountryAustralia
CityBrisbane
Period19/08/1922/08/19
Internet address

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feedlots
beef cattle
bovine respiratory disease
sampling
lungs
microbiome
Actinobacteria
cattle
Proteobacteria
digestive system diseases
economics
bacteria
DNA

Cite this

Maslen, B., Campbell, M., Ghorashi, S., & Pant, S. (2019). Temporal changes in gastrointestinal microbiomes of beef cattle on feedlot placement. 134. Abstract from 2019 Northern Beef Research Update Conference, Brisbane, Australia.
Maslen, Brianna ; Campbell, Michael ; Ghorashi, Seyed ; Pant, Sam. / Temporal changes in gastrointestinal microbiomes of beef cattle on feedlot placement. Abstract from 2019 Northern Beef Research Update Conference, Brisbane, Australia.1 p.
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abstract = "Introduction: A majority of beef cattle are finished in feedlots after purchase. However, feedlot placement has also been found to adversely influence cattle health, predisposing them to many diseases including Bovine Respiratory Disease(BRD). BRD is an important feedlot disease causing significant economic losses in beef cattle. While the influence of feedlot transition on disease predisposition is known to be mechanistically complex and multifactorial, the host microbiome could play a key role. Accordingly, the current project aimed to characterise temporal changes in gastrointestinal microbiota of beef cattle upon feedlot placement.Methods: Faecal samples from 30 cattle were sampled upon feedlot induction (day 0), and then repeat sampling was carried out on days 2, 7 and 14. Lung scores were obtained on the last day of sampling (day 14) and subsequently15 samples were chosen based on consistency of faecal samples across all four days of sampling, and lung scores obtained on day 14. Total DNA extracted from these faecal samples were subjected to 16s rDNA sequencing to determine the relative abundance of microbial species.Results: Results indicate that feedlot transition significantly impact gastrointestinal microbiome profiles, particularly around 2 days after feedlot placement. On day 2, the proportion of key groups of bacteria previously associated with gastrointestinal diseases, like proteobacteria and actinobacteria, were significantly affected. Conclusions: Overall, results suggest that gastrointestinal microbiota are significantly impacted due to feedlot placement of beef cattle, and their role in contributing to feedlot associated disease predisposition, including BRD, warrantsfurther investigated",
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Maslen, B, Campbell, M, Ghorashi, S & Pant, S 2019, 'Temporal changes in gastrointestinal microbiomes of beef cattle on feedlot placement', 2019 Northern Beef Research Update Conference, Brisbane, Australia, 19/08/19 - 22/08/19 pp. 134.

Temporal changes in gastrointestinal microbiomes of beef cattle on feedlot placement. / Maslen, Brianna; Campbell, Michael; Ghorashi, Seyed; Pant, Sam.

2019. 134 Abstract from 2019 Northern Beef Research Update Conference, Brisbane, Australia.

Research output: Other contribution to conferenceAbstract

TY - CONF

T1 - Temporal changes in gastrointestinal microbiomes of beef cattle on feedlot placement

AU - Maslen, Brianna

AU - Campbell, Michael

AU - Ghorashi, Seyed

AU - Pant, Sam

PY - 2019/8/21

Y1 - 2019/8/21

N2 - Introduction: A majority of beef cattle are finished in feedlots after purchase. However, feedlot placement has also been found to adversely influence cattle health, predisposing them to many diseases including Bovine Respiratory Disease(BRD). BRD is an important feedlot disease causing significant economic losses in beef cattle. While the influence of feedlot transition on disease predisposition is known to be mechanistically complex and multifactorial, the host microbiome could play a key role. Accordingly, the current project aimed to characterise temporal changes in gastrointestinal microbiota of beef cattle upon feedlot placement.Methods: Faecal samples from 30 cattle were sampled upon feedlot induction (day 0), and then repeat sampling was carried out on days 2, 7 and 14. Lung scores were obtained on the last day of sampling (day 14) and subsequently15 samples were chosen based on consistency of faecal samples across all four days of sampling, and lung scores obtained on day 14. Total DNA extracted from these faecal samples were subjected to 16s rDNA sequencing to determine the relative abundance of microbial species.Results: Results indicate that feedlot transition significantly impact gastrointestinal microbiome profiles, particularly around 2 days after feedlot placement. On day 2, the proportion of key groups of bacteria previously associated with gastrointestinal diseases, like proteobacteria and actinobacteria, were significantly affected. Conclusions: Overall, results suggest that gastrointestinal microbiota are significantly impacted due to feedlot placement of beef cattle, and their role in contributing to feedlot associated disease predisposition, including BRD, warrantsfurther investigated

AB - Introduction: A majority of beef cattle are finished in feedlots after purchase. However, feedlot placement has also been found to adversely influence cattle health, predisposing them to many diseases including Bovine Respiratory Disease(BRD). BRD is an important feedlot disease causing significant economic losses in beef cattle. While the influence of feedlot transition on disease predisposition is known to be mechanistically complex and multifactorial, the host microbiome could play a key role. Accordingly, the current project aimed to characterise temporal changes in gastrointestinal microbiota of beef cattle upon feedlot placement.Methods: Faecal samples from 30 cattle were sampled upon feedlot induction (day 0), and then repeat sampling was carried out on days 2, 7 and 14. Lung scores were obtained on the last day of sampling (day 14) and subsequently15 samples were chosen based on consistency of faecal samples across all four days of sampling, and lung scores obtained on day 14. Total DNA extracted from these faecal samples were subjected to 16s rDNA sequencing to determine the relative abundance of microbial species.Results: Results indicate that feedlot transition significantly impact gastrointestinal microbiome profiles, particularly around 2 days after feedlot placement. On day 2, the proportion of key groups of bacteria previously associated with gastrointestinal diseases, like proteobacteria and actinobacteria, were significantly affected. Conclusions: Overall, results suggest that gastrointestinal microbiota are significantly impacted due to feedlot placement of beef cattle, and their role in contributing to feedlot associated disease predisposition, including BRD, warrantsfurther investigated

KW - Beef cattle

KW - Microbiota

KW - Feedlots

KW - Bovine respiratory disease

M3 - Abstract

SP - 134

ER -

Maslen B, Campbell M, Ghorashi S, Pant S. Temporal changes in gastrointestinal microbiomes of beef cattle on feedlot placement. 2019. Abstract from 2019 Northern Beef Research Update Conference, Brisbane, Australia.