The consistency and influence of environmental and animal factors on exhaled breath condensate hydrogen peroxide, pH and leukotriene B4 in horses

S du Preez, S L Raidal, G S Doran, S G Nielsen, K J Hughes

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This study was performed to determine the consistency of exhaled breath condensate (EBC) hydrogen peroxide (H2O2), pH and leukotriene B4 (LTB4) measurements in asymptomatic horses and to define the influence of environmental and animal factors on these variables. Intra- and inter-day consistency for both H2O2 and pH measurements were adequate, with intraclass correlation coefficients ≥0.8, whereas the consistency for LTB4 was poor. H2O2 was influenced by ambient temperature (TA), humidity, time of day and collection location (all P<0.01), while pH was influenced by respiratory rate during EBC collection and TA (both P<0.001). The consistency of EBC H2O2 and pH measurements may be sufficient for use as diagnostic biomarkers in horses. However, the influence of identified environmental and animal factors should be considered.

Original languageEnglish
Pages (from-to)46-50
Number of pages5
JournalThe Veterinary Journal
Volume226
DOIs
Publication statusPublished - Aug 2017

Fingerprint

leukotrienes
Leukotriene B4
Hydrogen Peroxide
Horses
hydrogen peroxide
horses
animals
Temperature
Respiratory Rate
respiratory rate
Humidity
humidity
biomarkers
ambient temperature
Biomarkers
temperature

Cite this

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title = "The consistency and influence of environmental and animal factors on exhaled breath condensate hydrogen peroxide, pH and leukotriene B4 in horses",
abstract = "This study was performed to determine the consistency of exhaled breath condensate (EBC) hydrogen peroxide (H2O2), pH and leukotriene B4 (LTB4) measurements in asymptomatic horses and to define the influence of environmental and animal factors on these variables. Intra- and inter-day consistency for both H2O2 and pH measurements were adequate, with intraclass correlation coefficients ≥0.8, whereas the consistency for LTB4 was poor. H2O2 was influenced by ambient temperature (TA), humidity, time of day and collection location (all P<0.01), while pH was influenced by respiratory rate during EBC collection and TA (both P<0.001). The consistency of EBC H2O2 and pH measurements may be sufficient for use as diagnostic biomarkers in horses. However, the influence of identified environmental and animal factors should be considered.",
keywords = "EBC biomarker, Equine, H2O2, Leukotriene B4, Respiratory",
author = "{du Preez}, S and Raidal, {S L} and Doran, {G S} and Nielsen, {S G} and Hughes, {K J}",
note = "Includes bibliographical references.",
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language = "English",
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TY - JOUR

T1 - The consistency and influence of environmental and animal factors on exhaled breath condensate hydrogen peroxide, pH and leukotriene B4 in horses

AU - du Preez, S

AU - Raidal, S L

AU - Doran, G S

AU - Nielsen, S G

AU - Hughes, K J

N1 - Includes bibliographical references.

PY - 2017/8

Y1 - 2017/8

N2 - This study was performed to determine the consistency of exhaled breath condensate (EBC) hydrogen peroxide (H2O2), pH and leukotriene B4 (LTB4) measurements in asymptomatic horses and to define the influence of environmental and animal factors on these variables. Intra- and inter-day consistency for both H2O2 and pH measurements were adequate, with intraclass correlation coefficients ≥0.8, whereas the consistency for LTB4 was poor. H2O2 was influenced by ambient temperature (TA), humidity, time of day and collection location (all P<0.01), while pH was influenced by respiratory rate during EBC collection and TA (both P<0.001). The consistency of EBC H2O2 and pH measurements may be sufficient for use as diagnostic biomarkers in horses. However, the influence of identified environmental and animal factors should be considered.

AB - This study was performed to determine the consistency of exhaled breath condensate (EBC) hydrogen peroxide (H2O2), pH and leukotriene B4 (LTB4) measurements in asymptomatic horses and to define the influence of environmental and animal factors on these variables. Intra- and inter-day consistency for both H2O2 and pH measurements were adequate, with intraclass correlation coefficients ≥0.8, whereas the consistency for LTB4 was poor. H2O2 was influenced by ambient temperature (TA), humidity, time of day and collection location (all P<0.01), while pH was influenced by respiratory rate during EBC collection and TA (both P<0.001). The consistency of EBC H2O2 and pH measurements may be sufficient for use as diagnostic biomarkers in horses. However, the influence of identified environmental and animal factors should be considered.

KW - EBC biomarker

KW - Equine

KW - H2O2

KW - Leukotriene B4

KW - Respiratory

U2 - 10.1016/j.tvjl.2017.07.005

DO - 10.1016/j.tvjl.2017.07.005

M3 - Article

C2 - 28911841

VL - 226

SP - 46

EP - 50

JO - The Veterinary Journal

JF - The Veterinary Journal

SN - 1090-0233

ER -