The consistency and influence of environmental and animal factors on exhaled breath condensate hydrogen peroxide, pH and leukotriene B4 in horses

S du Preez, S L Raidal, G S Doran, S G Nielsen, K J Hughes

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2 Citations (Scopus)

Abstract

This study was performed to determine the consistency of exhaled breath condensate (EBC) hydrogen peroxide (H2O2), pH and leukotriene B4 (LTB4) measurements in asymptomatic horses and to define the influence of environmental and animal factors on these variables. Intra- and inter-day consistency for both H2O2 and pH measurements were adequate, with intraclass correlation coefficients ≥0.8, whereas the consistency for LTB4 was poor. H2O2 was influenced by ambient temperature (TA), humidity, time of day and collection location (all P<0.01), while pH was influenced by respiratory rate during EBC collection and TA (both P<0.001). The consistency of EBC H2O2 and pH measurements may be sufficient for use as diagnostic biomarkers in horses. However, the influence of identified environmental and animal factors should be considered.

Original languageEnglish
Pages (from-to)46-50
Number of pages5
JournalThe Veterinary Journal
Volume226
DOIs
Publication statusPublished - Aug 2017

Fingerprint

leukotrienes
Leukotriene B4
Hydrogen Peroxide
Horses
hydrogen peroxide
horses
animals
Temperature
Respiratory Rate
respiratory rate
Humidity
humidity
biomarkers
ambient temperature
Biomarkers
temperature

Cite this

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abstract = "This study was performed to determine the consistency of exhaled breath condensate (EBC) hydrogen peroxide (H2O2), pH and leukotriene B4 (LTB4) measurements in asymptomatic horses and to define the influence of environmental and animal factors on these variables. Intra- and inter-day consistency for both H2O2 and pH measurements were adequate, with intraclass correlation coefficients ≥0.8, whereas the consistency for LTB4 was poor. H2O2 was influenced by ambient temperature (TA), humidity, time of day and collection location (all P<0.01), while pH was influenced by respiratory rate during EBC collection and TA (both P<0.001). The consistency of EBC H2O2 and pH measurements may be sufficient for use as diagnostic biomarkers in horses. However, the influence of identified environmental and animal factors should be considered.",
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T1 - The consistency and influence of environmental and animal factors on exhaled breath condensate hydrogen peroxide, pH and leukotriene B4 in horses

AU - du Preez, S

AU - Raidal, S L

AU - Doran, G S

AU - Nielsen, S G

AU - Hughes, K J

N1 - Copyright © 2017 Elsevier Ltd. All rights reserved.

PY - 2017/8

Y1 - 2017/8

N2 - This study was performed to determine the consistency of exhaled breath condensate (EBC) hydrogen peroxide (H2O2), pH and leukotriene B4 (LTB4) measurements in asymptomatic horses and to define the influence of environmental and animal factors on these variables. Intra- and inter-day consistency for both H2O2 and pH measurements were adequate, with intraclass correlation coefficients ≥0.8, whereas the consistency for LTB4 was poor. H2O2 was influenced by ambient temperature (TA), humidity, time of day and collection location (all P<0.01), while pH was influenced by respiratory rate during EBC collection and TA (both P<0.001). The consistency of EBC H2O2 and pH measurements may be sufficient for use as diagnostic biomarkers in horses. However, the influence of identified environmental and animal factors should be considered.

AB - This study was performed to determine the consistency of exhaled breath condensate (EBC) hydrogen peroxide (H2O2), pH and leukotriene B4 (LTB4) measurements in asymptomatic horses and to define the influence of environmental and animal factors on these variables. Intra- and inter-day consistency for both H2O2 and pH measurements were adequate, with intraclass correlation coefficients ≥0.8, whereas the consistency for LTB4 was poor. H2O2 was influenced by ambient temperature (TA), humidity, time of day and collection location (all P<0.01), while pH was influenced by respiratory rate during EBC collection and TA (both P<0.001). The consistency of EBC H2O2 and pH measurements may be sufficient for use as diagnostic biomarkers in horses. However, the influence of identified environmental and animal factors should be considered.

KW - Journal Article

U2 - 10.1016/j.tvjl.2017.07.005

DO - 10.1016/j.tvjl.2017.07.005

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