The effect of active immunization against adrenocorticotropic hormone on cortisol, ß-endorphin, vocalization, and growth in pigs

C. Lee, L.R. Giles, W.L. Bryden, J.A. Downing, D.C. Collins, Peter Wynn

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Abstract

Because the poor growth performance of intensively housed pigs is associated with increased circulating glucocorticoid concentrations, we investigated the effects of glucocorticoid suppression by inducing a humoral immune response to ACTH on physiological and production variables in growing pigs. Grower pigs (28.6 ± 0.9 kg) were immunized with amino acids 1 through 24 of ACTH conjugated to ovalbumin and suspended in diethylaminoethyl (DEAE) dextran-adjuvant or adjuvant alone (control) on d 1, 28, and 56. The ACTH-specific antibody titers generated suppressed increases in cortisol concentrations on d 63 in response to an acute stressor (P = 0.002; control = 71 ± 8.2 ng/mL; ACTH-immune = 43 ± 4.9 ng/mL) without altering basal concentrations. Plasma ß-endorphin concentrations were also increased (P < 0.001) on d 63 (control = 18 ± 2.1 ng/mL; ACTH-immune = 63 ± 7.3 ng/mL), presumably because of a release from negative feedback on the expression of proopiomelanocortin in pituitary corticotropes. Immunization against ACTH did not alter ADG (P = 0.120; control = 1,077 ± 25; ACTH-immune = 1,143 ± 25 g) or ADFI (P = 0.64; control = 2,719 ± 42; ACTH-immune = 2,749 ± 42 g) and did not modify behavior (P = 0.681) assessed by measuring vocalization in response to acute restraint. In summary, suppression of stress-induced cortisol responses through ACTH immunization increased ß-endorphin concentrations, but it did not modify ADG, ADFI, or restraint vocalization score in growing pigs.
Original languageEnglish
Pages (from-to)2372-2379
Number of pages8
JournalJournal of Animal Science
Volume83
Issue number10
Publication statusPublished - 2005

Fingerprint

Endorphins
endorphins
corticotropin
vocalization
Adrenocorticotropic Hormone
cortisol
Hydrocortisone
immunization
Vaccination
Swine
swine
Growth
Glucocorticoids
glucocorticoids
Immunization
adjuvants
Cosyntropin
Pro-Opiomelanocortin
pro-opiomelanocortin
feeder pigs

Cite this

Lee, C. ; Giles, L.R. ; Bryden, W.L. ; Downing, J.A. ; Collins, D.C. ; Wynn, Peter. / The effect of active immunization against adrenocorticotropic hormone on cortisol, ß-endorphin, vocalization, and growth in pigs. In: Journal of Animal Science. 2005 ; Vol. 83, No. 10. pp. 2372-2379.
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abstract = "Because the poor growth performance of intensively housed pigs is associated with increased circulating glucocorticoid concentrations, we investigated the effects of glucocorticoid suppression by inducing a humoral immune response to ACTH on physiological and production variables in growing pigs. Grower pigs (28.6 ± 0.9 kg) were immunized with amino acids 1 through 24 of ACTH conjugated to ovalbumin and suspended in diethylaminoethyl (DEAE) dextran-adjuvant or adjuvant alone (control) on d 1, 28, and 56. The ACTH-specific antibody titers generated suppressed increases in cortisol concentrations on d 63 in response to an acute stressor (P = 0.002; control = 71 ± 8.2 ng/mL; ACTH-immune = 43 ± 4.9 ng/mL) without altering basal concentrations. Plasma {\~A}Ÿ-endorphin concentrations were also increased (P < 0.001) on d 63 (control = 18 ± 2.1 ng/mL; ACTH-immune = 63 ± 7.3 ng/mL), presumably because of a release from negative feedback on the expression of proopiomelanocortin in pituitary corticotropes. Immunization against ACTH did not alter ADG (P = 0.120; control = 1,077 ± 25; ACTH-immune = 1,143 ± 25 g) or ADFI (P = 0.64; control = 2,719 ± 42; ACTH-immune = 2,749 ± 42 g) and did not modify behavior (P = 0.681) assessed by measuring vocalization in response to acute restraint. In summary, suppression of stress-induced cortisol responses through ACTH immunization increased {\~A}Ÿ-endorphin concentrations, but it did not modify ADG, ADFI, or restraint vocalization score in growing pigs.",
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The effect of active immunization against adrenocorticotropic hormone on cortisol, ß-endorphin, vocalization, and growth in pigs. / Lee, C.; Giles, L.R.; Bryden, W.L.; Downing, J.A.; Collins, D.C.; Wynn, Peter.

In: Journal of Animal Science, Vol. 83, No. 10, 2005, p. 2372-2379.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The effect of active immunization against adrenocorticotropic hormone on cortisol, ß-endorphin, vocalization, and growth in pigs

AU - Lee, C.

AU - Giles, L.R.

AU - Bryden, W.L.

AU - Downing, J.A.

AU - Collins, D.C.

AU - Wynn, Peter

N1 - Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = Journal of Animal Science. ISSNs: 0021-8812;

PY - 2005

Y1 - 2005

N2 - Because the poor growth performance of intensively housed pigs is associated with increased circulating glucocorticoid concentrations, we investigated the effects of glucocorticoid suppression by inducing a humoral immune response to ACTH on physiological and production variables in growing pigs. Grower pigs (28.6 ± 0.9 kg) were immunized with amino acids 1 through 24 of ACTH conjugated to ovalbumin and suspended in diethylaminoethyl (DEAE) dextran-adjuvant or adjuvant alone (control) on d 1, 28, and 56. The ACTH-specific antibody titers generated suppressed increases in cortisol concentrations on d 63 in response to an acute stressor (P = 0.002; control = 71 ± 8.2 ng/mL; ACTH-immune = 43 ± 4.9 ng/mL) without altering basal concentrations. Plasma ß-endorphin concentrations were also increased (P < 0.001) on d 63 (control = 18 ± 2.1 ng/mL; ACTH-immune = 63 ± 7.3 ng/mL), presumably because of a release from negative feedback on the expression of proopiomelanocortin in pituitary corticotropes. Immunization against ACTH did not alter ADG (P = 0.120; control = 1,077 ± 25; ACTH-immune = 1,143 ± 25 g) or ADFI (P = 0.64; control = 2,719 ± 42; ACTH-immune = 2,749 ± 42 g) and did not modify behavior (P = 0.681) assessed by measuring vocalization in response to acute restraint. In summary, suppression of stress-induced cortisol responses through ACTH immunization increased ß-endorphin concentrations, but it did not modify ADG, ADFI, or restraint vocalization score in growing pigs.

AB - Because the poor growth performance of intensively housed pigs is associated with increased circulating glucocorticoid concentrations, we investigated the effects of glucocorticoid suppression by inducing a humoral immune response to ACTH on physiological and production variables in growing pigs. Grower pigs (28.6 ± 0.9 kg) were immunized with amino acids 1 through 24 of ACTH conjugated to ovalbumin and suspended in diethylaminoethyl (DEAE) dextran-adjuvant or adjuvant alone (control) on d 1, 28, and 56. The ACTH-specific antibody titers generated suppressed increases in cortisol concentrations on d 63 in response to an acute stressor (P = 0.002; control = 71 ± 8.2 ng/mL; ACTH-immune = 43 ± 4.9 ng/mL) without altering basal concentrations. Plasma ß-endorphin concentrations were also increased (P < 0.001) on d 63 (control = 18 ± 2.1 ng/mL; ACTH-immune = 63 ± 7.3 ng/mL), presumably because of a release from negative feedback on the expression of proopiomelanocortin in pituitary corticotropes. Immunization against ACTH did not alter ADG (P = 0.120; control = 1,077 ± 25; ACTH-immune = 1,143 ± 25 g) or ADFI (P = 0.64; control = 2,719 ± 42; ACTH-immune = 2,749 ± 42 g) and did not modify behavior (P = 0.681) assessed by measuring vocalization in response to acute restraint. In summary, suppression of stress-induced cortisol responses through ACTH immunization increased ß-endorphin concentrations, but it did not modify ADG, ADFI, or restraint vocalization score in growing pigs.

KW - Adrenocorticotropic Hormone Immunization

KW - Cortisol

KW - Growth

KW - Pig

KW - Vocalization

KW - ß-Endorphin

M3 - Article

VL - 83

SP - 2372

EP - 2379

JO - J Anim Sci

JF - J Anim Sci

SN - 0021-8812

IS - 10

ER -