The effect of storage time and temperature on interpretation of protein concentration and cellular features of equine body fluids

Kris Hughes, S. Higgins, D.I Rendle, D.J. Mellor, R. Barron, Sandy Love, A.E Durham

Research output: Other contribution to conferenceAbstractpeer-review

Abstract

Aims: To assess the effects of storage time and temperature on protein and cell parameters of body fluids collected from horses and determine whether any changes affect clinicopathological interpretation. Methods: Body fluid samples were obtained from horses during diagnostic investigation, divided into 7 aliquots and subjected to laboratory examination immediately (T0), or 24 hours (T24), T48 and T72 after collection and stored at 4oC or 22oC. Total protein concentration (TP), total nucleated cell count (TNCC) and cytological findings were recorded for each aliquot. Neutrophil degeneration was graded using an ordinal scale. Paired t-tests were used to analyse TP as the data were normally distributed. TNCC data were normalised by log transformation prior to t-test analysis. Investigations of changes in median neutrophil morphology grade between time points at storage temperatures were performed using a non-parametric one sample Wilcoxon test. Results: 33 samples (peritoneal fluid, n=18; synovial fluid, n=11; pleural fluid, n=3; CSF, n=1) were collected from 24 horses. No significant difference in TP occurred between T0 and any of the other time periods for samples stored at either 4oC or 22oC. Compared to T0, TNCC was significantly lower at T72 at 4oC and T24, T48 and T72 at 22oC (P<0.005). Neutrophil degeneration score was increased significantly from T0 at T24, T48 and T72 at both 4oC and 22oC (P<0.005). At T24, clinicopathological interpretation was different for 3 and 4 samples stored at 4oC and 22oC, respectively. At T72, clinicopathological interpretation was different for 5 and 6 samples stored at 4oC and 22oC, respectively. Conclusions and practical significance: Alterations in the TNCC and cytology of body fluid samples occur when laboratory analysis is delayed. Such alterations may influence laboratory interpretation and clinical decision making. Fluid samples collected from body cavities should be analysed as soon as possible after collection.
Original languageEnglish
Pages309-309
Number of pages1
Publication statusPublished - 2007
Event46th British Equine Veterinary Association Congress - Edinburgh, United Kingdom
Duration: 12 Sep 200715 Sep 2007

Conference

Conference46th British Equine Veterinary Association Congress
Country/TerritoryUnited Kingdom
CityEdinburgh
Period12/09/0715/09/07

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