The ex vivo antiplatelet activation potential of fruit phenolic metabolite hippuric acid

Abishek Bommannan Santhakumar; Roger Stanley; Indu Singh

doi:10.1039/c5fo00715a

The ex vivo antiplatelet activation potential of fruit phenolic metabolite hippuric acid

Abishek Bommannan Santhakumar, Roger Stanley, Indu Singh

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Polyphenol-rich fruit and vegetable intake has been associated with reduction in platelet hyperactivity, a significant contributor to thrombus formation. This study was undertaken to investigate the possible role of hippuric acid, a predominant metabolite of plant cyclic polyols, phenolic acids and polyphenols, in reduction of platelet activation-related thrombogenesis. Fasting blood samples were collected from 13 healthy subjects to analyse the effect of varying concentrations of hippuric acid (100 μM, 200 μM, 500 μM, 1 mM and 2 mM) on activation-dependant platelet surface-marker expression. Procaspase activating compound-1 (PAC-1) and P-selectin/CD62P monoclonal antibodies were used to evaluate platelet activation-related conformational changes and α-granule release respectively using flow cytometry. Platelets were stimulated ex vivo via the P2Y1/P2Y12 – adenosine diphosphate (ADP) pathway of platelet activation.

Original language	English
Pages (from-to)	2679-2683
Number of pages	5
Journal	Food and Function
Volume	6
DOIs	https://doi.org/10.1039/c5fo00715a
Publication status	Published - 2015

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title = "The ex vivo antiplatelet activation potential of fruit phenolic metabolite hippuric acid",

abstract = "Polyphenol-rich fruit and vegetable intake has been associated with reduction in platelet hyperactivity, a significant contributor to thrombus formation. This study was undertaken to investigate the possible role of hippuric acid, a predominant metabolite of plant cyclic polyols, phenolic acids and polyphenols, in reduction of platelet activation-related thrombogenesis. Fasting blood samples were collected from 13 healthy subjects to analyse the effect of varying concentrations of hippuric acid (100 μM, 200 μM, 500 μM, 1 mM and 2 mM) on activation-dependant platelet surface-marker expression. Procaspase activating compound-1 (PAC-1) and P-selectin/CD62P monoclonal antibodies were used to evaluate platelet activation-related conformational changes and α-granule release respectively using flow cytometry. Platelets were stimulated ex vivo via the P2Y1/P2Y12 – adenosine diphosphate (ADP) pathway of platelet activation. ",

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AU - Santhakumar, Abishek Bommannan

AU - Stanley, Roger

AU - Singh, Indu

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AB - Polyphenol-rich fruit and vegetable intake has been associated with reduction in platelet hyperactivity, a significant contributor to thrombus formation. This study was undertaken to investigate the possible role of hippuric acid, a predominant metabolite of plant cyclic polyols, phenolic acids and polyphenols, in reduction of platelet activation-related thrombogenesis. Fasting blood samples were collected from 13 healthy subjects to analyse the effect of varying concentrations of hippuric acid (100 μM, 200 μM, 500 μM, 1 mM and 2 mM) on activation-dependant platelet surface-marker expression. Procaspase activating compound-1 (PAC-1) and P-selectin/CD62P monoclonal antibodies were used to evaluate platelet activation-related conformational changes and α-granule release respectively using flow cytometry. Platelets were stimulated ex vivo via the P2Y1/P2Y12 – adenosine diphosphate (ADP) pathway of platelet activation.

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DO - 10.1039/c5fo00715a

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The ex vivo antiplatelet activation potential of fruit phenolic metabolite hippuric acid

Abstract

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