The haemagglutination spectrum of psittacine beak and feather disease virus

S. R. Raidal, G. M. Cross

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

A simple method for concentrating psittacine beak and feather disease virus (PBFDV) from crude feather suspensions is described. The addition of 10% polyethylene glycol (MW 6000 to 9000) to feather suspensions facilitated the precipitation and pelleting of PBFDV by low speed centrifugation. Pellets were resuspended in one-twentieth of the original volume with caesium chloride (CsCl) buffer and subjected to isopycnic ultracentrifugation. Peak haemagglutination activity (HA) occurred at 1.35 g/ml in PBFDV CsCl gradients. CsCl purified virus agglutinated galah (Eolophus roseicapillus), eastern long-billed corella (Cacatua tenuirostris), sulphur-crested cockatoo (Cacatua galerita), Major Mitchell's cockatoo (Cacatua lead-beaten) and gang gang cockatoo (Callocephalon fimbriatum) erythrocytes, but not those of 19 other avian or five mammalian species. PBFDV agglutinated galah erythrocytes at 4°C and 37°C over a wide range of pH and no change in HA titre was observed when PBFDV was treated with chloroform. HA persisted in PBFDV suspensions heated to 80°C for 30 min, but was not detected after incubation at higher temperatures. High HA titres were detected in the feathers, serum, liver and kidneys of PBFD-affected birds.

Original languageEnglish
Pages (from-to)621-630
Number of pages10
JournalAvian Pathology
Volume23
Issue number4
DOIs
Publication statusPublished - 01 Dec 1994

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