The Immunohistochemical characterization of devil facial tumor disease (DFTD) in the Tasmanian Devil (Sarcophilus harrisii)

R. Loh, D. Hayes, A. Mahjoor, A. O'Hara, S. Pyecroft, Shane Raidal

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Abstract

Immunohistochemical techniques were used to characterize the disfiguring and debilitating fatal neoplastic disease, devil facial tumor disease (DFTD), which has recently affected a significant proportion of the wild population of Tasmanian Devils (Sarcophilus harrisii). The diagnostic values of a number of immunohistochemical stains were employed to further characterize 50 representative cases. The neoplasms were negative for cytokeratin (0/48), epithelial membrane antigen (0/42), von Willebrand factor (vWF) (0/11), smooth muscle actin (SMA) (0/26), desmin (0/47), glial fibrillary acid protein (0/13), CD16 (0/13), CD57 (0/43), CD3 (0/18), and LSP1 (0/16). DFTD cells were positive for vimentin (50/50), S-100 (41/48), melan A (11/39), neuron specific enolase (35/35), chromogranin A (12/12) and synaptophysin (29/30). The cells were negative for amyloid (0/30) and stained negatively with Singh's silver (0/34) but were weakly argyrophilic (3/40) using Grimelius histochemical stain. These staining characteristics are consistent with cells of neuroectodermal origin.
Original languageEnglish
Pages (from-to)896-903
Number of pages8
JournalVeterinary Pathology
Volume43
Issue number6
DOIs
Publication statusPublished - 2006

Fingerprint

neoplasms
dyes
MART-1 Antigen
Chromogranin A
Mucin-1
Neoplasms
desmin
blood coagulation factors
Synaptophysin
phosphopyruvate hydratase
Desmin
Phosphopyruvate Hydratase
vimentin
Glial Fibrillary Acidic Protein
von Willebrand Factor
neuroglia
Vimentin
disease diagnosis
cells
amyloid

Cite this

Loh, R. ; Hayes, D. ; Mahjoor, A. ; O'Hara, A. ; Pyecroft, S. ; Raidal, Shane. / The Immunohistochemical characterization of devil facial tumor disease (DFTD) in the Tasmanian Devil (Sarcophilus harrisii). In: Veterinary Pathology. 2006 ; Vol. 43, No. 6. pp. 896-903.
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abstract = "Immunohistochemical techniques were used to characterize the disfiguring and debilitating fatal neoplastic disease, devil facial tumor disease (DFTD), which has recently affected a significant proportion of the wild population of Tasmanian Devils (Sarcophilus harrisii). The diagnostic values of a number of immunohistochemical stains were employed to further characterize 50 representative cases. The neoplasms were negative for cytokeratin (0/48), epithelial membrane antigen (0/42), von Willebrand factor (vWF) (0/11), smooth muscle actin (SMA) (0/26), desmin (0/47), glial fibrillary acid protein (0/13), CD16 (0/13), CD57 (0/43), CD3 (0/18), and LSP1 (0/16). DFTD cells were positive for vimentin (50/50), S-100 (41/48), melan A (11/39), neuron specific enolase (35/35), chromogranin A (12/12) and synaptophysin (29/30). The cells were negative for amyloid (0/30) and stained negatively with Singh's silver (0/34) but were weakly argyrophilic (3/40) using Grimelius histochemical stain. These staining characteristics are consistent with cells of neuroectodermal origin.",
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The Immunohistochemical characterization of devil facial tumor disease (DFTD) in the Tasmanian Devil (Sarcophilus harrisii). / Loh, R.; Hayes, D.; Mahjoor, A.; O'Hara, A.; Pyecroft, S.; Raidal, Shane.

In: Veterinary Pathology, Vol. 43, No. 6, 2006, p. 896-903.

Research output: Contribution to journalArticle

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T1 - The Immunohistochemical characterization of devil facial tumor disease (DFTD) in the Tasmanian Devil (Sarcophilus harrisii)

AU - Loh, R.

AU - Hayes, D.

AU - Mahjoor, A.

AU - O'Hara, A.

AU - Pyecroft, S.

AU - Raidal, Shane

N1 - Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = Veterinary Pathology. ISSNs: 0300-9858;

PY - 2006

Y1 - 2006

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AB - Immunohistochemical techniques were used to characterize the disfiguring and debilitating fatal neoplastic disease, devil facial tumor disease (DFTD), which has recently affected a significant proportion of the wild population of Tasmanian Devils (Sarcophilus harrisii). The diagnostic values of a number of immunohistochemical stains were employed to further characterize 50 representative cases. The neoplasms were negative for cytokeratin (0/48), epithelial membrane antigen (0/42), von Willebrand factor (vWF) (0/11), smooth muscle actin (SMA) (0/26), desmin (0/47), glial fibrillary acid protein (0/13), CD16 (0/13), CD57 (0/43), CD3 (0/18), and LSP1 (0/16). DFTD cells were positive for vimentin (50/50), S-100 (41/48), melan A (11/39), neuron specific enolase (35/35), chromogranin A (12/12) and synaptophysin (29/30). The cells were negative for amyloid (0/30) and stained negatively with Singh's silver (0/34) but were weakly argyrophilic (3/40) using Grimelius histochemical stain. These staining characteristics are consistent with cells of neuroectodermal origin.

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KW - Tasmanian Devils

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