Abstract
Objectives
To molecular characterize the expression levels of lactoferrin receptor (LfR) in different regions of brain and its response to lactoferrin (LF) intervention in piglets.
Methods
3-day-old male piglets were randomly allocated to groups (Grp) 1 fed milk replacer supplemented with LF 1.1 g/L (n = 16) and Grp 2 0.06 g/L (n = 16) as control. Piglets were euthanised at 38 days of age. Gene and protein expression of LfR was analysed using we published methods (1). Study protocol has been approved by animal ethic committee of Xiamen University.
Results
Our results showed that the cellular levels of mRNA coding for LfR was differentially expressed in ten sub-regions of piglet's brain. The highest expression level of LfR gene was found in the prefrontal cortex (PF, 20.45 ± 2.11) following by the parietal lobe (PL), brainstem (BS), occipital lobe (OL), sub ventricular zone (SVZ), olfactory bulb (OB), hippocampus (Hip), amygdala (Amy), cerebellum (CE) and thalamus (THA) respectively. The mRNA expression of LfR in the PF was ∼15% time higher than that of thalamus (THA, 1.38 ± 0.549). Overall differences between sub-regions of brain were highly significant. There was a positive correlation between expression level of mRNA and protein of the tested tissues. LfR expression in different regions of brain significantly responded to dietary LF intervention in piglets (P < 0.05).
Conclusions
We demonstrated that LfR differentially expressed in different regions of brain and responded to dietary LF intervention in pigleta, an animal model of human infant. The cellular abundance of LfR in ten subregions of piglet brain is regulated at the level of transcription. The role of LfR in neurodevelopment is under investigation.
Funding Sources
Medical School of Xiamen University.
| Original language | English |
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| Pages | 1240 |
| Number of pages | 1 |
| DOIs | |
| Publication status | Published - 29 May 2020 |
| Event | Nutrition 2020 Live Online - Online Duration: 01 Jun 2020 → 04 Jun 2020 https://journals.lww.com/nutritiontodayonline/Citation/2020/07000/American_Society_for_Nutrition_Reinvents_NUTRITION.1.aspx https://academic.oup.com/cdn/issue/4/Supplement_2?page=9 (Published abstracts) |
Conference
| Conference | Nutrition 2020 Live Online |
|---|---|
| Period | 01/06/20 → 04/06/20 |
| Other | NUTRITION 2020 LIVE ONLINE emerged as a new event when the American Society for Nutrition (ASN) canceled its flagship in-person meeting, Nutrition 2020, in response to COVID-19 concerns. The organization says this about the new event: “With a high number of abstract submissions and registration numbers that put ASN on track to have the largest meeting in its history, ASN’s Board of Directors and leadership team has remained committed to delivering an experience the nutrition community craves.” |
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