The polymerase chain reaction (PCR): General methods

Daniel L E Waters, Frances M. Shapter

    Research output: Book chapter/Published conference paperChapter in textbook/reference bookpeer-review

    16 Citations (Scopus)

    Abstract

    The polymerase chain reaction (PCR) converts very low quantities of DNA into very high quantities and is the foundation of many specialized techniques of molecular biology. PCR utilizes components of the cellular machinery of mitotic cell division in vitro which respond predictably to user inputs. This chapter introduces the principles of PCR and discusses practical considerations from target sequence definition through to optimization and application.
    Original languageEnglish
    Title of host publicationCereal genomics
    Subtitle of host publicationMethods and protocols
    EditorsRobert J. Henry, Agnelo Furtado
    Place of PublicationNew York
    PublisherHumana Press Inc.
    Chapter7
    Pages65-75
    Number of pages11
    ISBN (Electronic)9781627037150
    ISBN (Print)9781627037143
    DOIs
    Publication statusPublished - 2014

    Publication series

    NameMethods in Molecular Biology
    Volume1099
    ISSN (Print)1064-3745

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