Abstract
The polymerase chain reaction (PCR) converts very low quantities of DNA into very high quantities and is the foundation of many specialized techniques of molecular biology. PCR utilizes components of the cellular machinery of mitotic cell division in vitro which respond predictably to user inputs. This chapter introduces the principles of PCR and discusses practical considerations from target sequence definition through to optimization and application.
| Original language | English |
|---|---|
| Title of host publication | Cereal genomics |
| Subtitle of host publication | Methods and protocols |
| Editors | Robert J. Henry, Agnelo Furtado |
| Place of Publication | New York |
| Publisher | Humana Press Inc. |
| Chapter | 7 |
| Pages | 65-75 |
| Number of pages | 11 |
| ISBN (Electronic) | 9781627037150 |
| ISBN (Print) | 9781627037143 |
| DOIs | |
| Publication status | Published - 2014 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1099 |
| ISSN (Print) | 1064-3745 |
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Cite this
Waters, D. L. E., & Shapter, F. M. (2014). The polymerase chain reaction (PCR): General methods. In R. J. Henry, & A. Furtado (Eds.), Cereal genomics: Methods and protocols (pp. 65-75). (Methods in Molecular Biology; Vol. 1099). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-715-0_7