The polymerase chain reaction (PCR): General methods

Daniel L E Waters, Frances M. Shapter

Research output: Book chapter/Published conference paperChapter in textbook/reference book

6 Citations (Scopus)

Abstract

The polymerase chain reaction (PCR) converts very low quantities of DNA into very high quantities and is the foundation of many specialized techniques of molecular biology. PCR utilizes components of the cellular machinery of mitotic cell division in vitro which respond predictably to user inputs. This chapter introduces the principles of PCR and discusses practical considerations from target sequence definition through to optimization and application.
Original languageEnglish
Title of host publicationCereal genomics
Subtitle of host publicationMethods and protocols
EditorsRobert J. Henry, Agnelo Furtado
Place of PublicationNew York
PublisherHumana Press Inc.
Chapter7
Pages65-75
Number of pages11
ISBN (Electronic)9781627037150
ISBN (Print)9781627037143
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1099
ISSN (Print)1064-3745

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    Waters, D. L. E., & Shapter, F. M. (2014). The polymerase chain reaction (PCR): General methods. In R. J. Henry, & A. Furtado (Eds.), Cereal genomics: Methods and protocols (pp. 65-75). (Methods in Molecular Biology; Vol. 1099). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-715-0_7