The polymerase chain reaction (PCR): General methods

Daniel L E Waters, Frances M. Shapter

    Research output: Book chapter/Published conference paperChapter in textbook/reference bookpeer-review

    26 Citations (Scopus)


    The polymerase chain reaction (PCR) converts very low quantities of DNA into very high quantities and is the foundation of many specialized techniques of molecular biology. PCR utilizes components of the cellular machinery of mitotic cell division in vitro which respond predictably to user inputs. This chapter introduces the principles of PCR and discusses practical considerations from target sequence definition through to optimization and application.
    Original languageEnglish
    Title of host publicationCereal genomics
    Subtitle of host publicationMethods and protocols
    EditorsRobert J. Henry, Agnelo Furtado
    Place of PublicationNew York
    PublisherHumana Press Inc.
    Number of pages11
    ISBN (Electronic)9781627037150
    ISBN (Print)9781627037143
    Publication statusPublished - 2014

    Publication series

    NameMethods in Molecular Biology
    ISSN (Print)1064-3745


    Dive into the research topics of 'The polymerase chain reaction (PCR): General methods'. Together they form a unique fingerprint.

    Cite this