Dromiciops gliroides is a small nocturnal marsupial found in the temperate forests of Southern Chile and Argentina. It is the only living member of the order Microbiotheria. Here, we describe the discovery and first account of live trapping of D. gliroides in Parque Nacional Los Alerces (Los Alerces National Park) in the province of Chubut, Argentina. This account extends the distribution of this cryptic species south to Parque Nacional Los Alerces (PNLA). The study provides a description of the habitat they were captured in after a recent bamboo flowering and seeding event and subsequent rodent irruption, the first in 70 years in PNLA.