TY - JOUR
T1 - The protective effect of 17β-estradiol against hydrogen peroxide-induced apoptosis on mesenchymal stem cell
AU - Chen, Hai-Ying
AU - Zhang, Xian
AU - Chen, Shuang-Feng
AU - Zhang, Ying-Xin
AU - Liu, Yi-Hua
AU - Ma, Longle
AU - Wang, Lexin
N1 - Imported on 12 Apr 2017 - DigiTool details were: month (773h) = February, 2012; Journal title (773t) = Biomedicine and Pharmacotherapy. ISSNs: 0753-3322;
PY - 2012/2
Y1 - 2012/2
N2 - This study was designed to investigate the function of 17ß-estradiol (17ß-E2) against oxidative stress on the cell death of mice bone marrow mesenchymal stem cells (BMSCs) induced by hydrogen peroxide (H2O2). BMSCs were treated with 17ß-E2 for 24 h and then treated with 100 'M H2O2 for 1 h. Cell viability, apoptosis, caspase-9 mRNA, JNKs (Jun N-terminal kinases) and c-Jun protein expression in BMSCs were evaluated. Cell apoptosis of BMSCs were increased in a dose-dependent manner after treated with H2O2 compared to control group. But pretreatment with 17ß-E2 can inhibit apoptosis of BMSCs, preserve the mitochondrial transmembrane potential, decrease caspase-9 mRNA, JNK1/2 and c-Jun protein expression. In conclusion, 17ß-E2 exerts antiapoptotic effects in BMSCs which related to the mitochondria death pathway and JNKs pathway. The study revealed that 17ß-E2 can reduce the donor stem cells damage.
AB - This study was designed to investigate the function of 17ß-estradiol (17ß-E2) against oxidative stress on the cell death of mice bone marrow mesenchymal stem cells (BMSCs) induced by hydrogen peroxide (H2O2). BMSCs were treated with 17ß-E2 for 24 h and then treated with 100 'M H2O2 for 1 h. Cell viability, apoptosis, caspase-9 mRNA, JNKs (Jun N-terminal kinases) and c-Jun protein expression in BMSCs were evaluated. Cell apoptosis of BMSCs were increased in a dose-dependent manner after treated with H2O2 compared to control group. But pretreatment with 17ß-E2 can inhibit apoptosis of BMSCs, preserve the mitochondrial transmembrane potential, decrease caspase-9 mRNA, JNK1/2 and c-Jun protein expression. In conclusion, 17ß-E2 exerts antiapoptotic effects in BMSCs which related to the mitochondria death pathway and JNKs pathway. The study revealed that 17ß-E2 can reduce the donor stem cells damage.
KW - 17Ã-Estradiol
KW - Bone marrow mesenchymal stem cells
KW - Hydrogen peroxide
KW - Jun N-terminal kinases
U2 - 10.1016/j.biopha.2011.11.014
DO - 10.1016/j.biopha.2011.11.014
M3 - Article
C2 - 22281292
SN - 0753-3322
VL - 66
SP - 57
EP - 63
JO - Biomedicine
JF - Biomedicine
IS - 1
ER -