The ripening wine grape berry skin transcriptome

Daniel L E Waters, Timothy A. Holton, Effie M. Ablett, L. Slade Lee, Robert J. Henry

    Research output: Contribution to journalArticlepeer-review

    24 Citations (Scopus)


    Ripening of the grape berry immediately precedes harvesting and is the key phase which determines the composition of wine and table grapes. Although it is apparent that changes in gene expression levels play a role in initiating ripening, it is unknown to what extent gene expression levels differ between cultivars during ripening. We have undertaken a comparison of a selection of wine grape cultivars using a 9200 feature cDNA microarray to gauge the extent to which transcript levels differ between wine grape cultivars in the berry skin during ripening. Clones for microarray slide fabrication were sourced from a cDNA library constructed from mRNA derived from randomly sampled berries. The berries were collected at weekly intervals over four weeks of berry development including véraison. The microarray slides were hybridised with Cy3 and Cy5 labelled cDNA derived from the skin of ripening berries of the cultivars Cabernet Sauvignon, Pinot Noir, Shiraz, Chardonnay, Riesling, Sauvignon Blanc and Semillon. Only a small proportion of the genes within the berry skin showed a three-fold or greater difference in expression level after ripening commenced. Most of the differences appear to arise from environmental signals rather than genome differences.

    Original languageEnglish
    Pages (from-to)132-138
    Number of pages7
    JournalPlant Science
    Issue number1
    Publication statusPublished - Jul 2006


    Dive into the research topics of 'The ripening wine grape berry skin transcriptome'. Together they form a unique fingerprint.

    Cite this